Cloned genes encoding reverse transcriptase lacking RNase H acti

Details Cloned genes encoding reverse transcriptase lacking RNase H acti Cloned genes encoding reverse transcriptase lacking RNase H acti

1997-02-10

2000-05-16

Achutamurthy, Ponnathapu

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435 691, 435 911, 435 912, 4352523, 43525233, 4353201, 435475, 435975, 536 232, C12N 912, C12N 1554, C12N 1570, C12P 1934

Patent

active

060636084

ABSTRACT:
The invention relates to a gene which encodes reverse transcriptase having DNA polymerase activity and substantially no RNase H activity. The invention also relates to vectors containing the gene and hosts transformed with the vectors of the invention. The invention also relates to a method of producing reverse transcriptase having DNA polymerase activity and substantially no RNase H activity by expressing the reverse transcriptase genes of the present invention in a host. The invention also relates to a method of producing cDNA from mRNA using the reverse transcriptase of the invention. The invention also relates to a kit for the preparation of cDNA from mRNA comprising the reverse transcriptase of the invention.

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