Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-07
2000-09-12
Ulm, John
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4352523, 4353201, 530350, 536 234, C07K 1900, C12N 1562
Patent
active
061176567
ABSTRACT:
The invention relates to a fusion protein which comprises an immunoglobulin of the IgM, IgG1 or IgG3 immunoglobulin class, wherein the variable region of the light or heavy chain has been replaced with CD4 or fragment thereof which is capable of binding to gp120. The invention also relates to an immunoglobulin-like molecule comprising the fusion protein of the invention together with an immunoglobulin light or heavy chain. The invention also relates to a method of treating HIV or SIV infection comprising administering the fusion proteins or immunoglobulin-like molecules of the invention to an animal. The invention also relates to assays for HIV or SIV comprising contacting a sample suspected of containing HIV or SIV gp120 with the immunoglobulin-like molecule of fusion protein of the invention, and detecting whether a complex is formed.
REFERENCES:
patent: 4675382 (1987-06-01), Murphy
patent: 4816567 (1989-03-01), Cabilly et al.
patent: 5234905 (1993-08-01), Kolhouse et al.
patent: 5336603 (1994-08-01), Capon et al.
Mizukami, T., et al., "Expression and Characterization of Chimeric Proteins Containing Human CD4 Linked to Human Immunoglobulin Heavy Chain Constant Regions," in Morisset, R.A. (ed.) V International Conference on AIDS, Abstract No. M.C.P. 89 (Montreal, Canada 1989), p. 556.
Stricker, et al., "An AIDS-related Cytotoxic Autoantibody Reacts with a Specific Antigen on Stimulated CD4+ T Cells," Nature 327:710-713 (1987).
Smith, D. H. et al., "Blocking of HIV-1 Infectivity by a Soluble, Secreted Form of the CD4 Antigen," Science 238:1704-1707 (1987).
Estess, P. et al., "Analysis of T-Cell Receptor Structure and Function Using Chimeric T-Cell Receptor/Immunoglobulin Molecules," J. Cell. Biochem. (Suppl. 11d) :Abstract 331, p. 258 (1987).
Clark, S. et al., "Peptide and nucleotide sequences of rat CD4 (W3/25) antigen: Evidence for derivation from a structure with four immunoglobulin-related domains," Proc. Natl. Acad. Sci. (USA) 84:1649-53 (1987).
Palker, T. J. et al., "A Conserved Region at the COOH Terminus of Human Immunodeficiency Virus gp120 Envelope Protein Contains an Immunodominant Epitope," Proc. Natl. Acad. Sci. (USA) 84:2479-2483 (1987).
Neuberger, M. S. et al., "Recombinant Antibodies Possessing Novel Effector Functions," Nature 312:604-608 (1984).
Rusche, J. A. et al., "Antibodies that Inhibit Fusion of Human Immunodeficiency Virus-Infected Cells Bind a 24-Amino Acid Sequence of the Viral Envelope, gp120," Proc. Natl. Acad. Sci. (USA) 85:3198-3202 (1988).
Boulianne, G. L. et al., "Production of Functional Chimaeric Mouse/Human Antibody," Nature 312:643-646 (1984).
Morrison, et al., "Chimeric human antibody molecules: Mouse antigen-binding domains with human constant region domains," Proc. Natl. Acad. Sci. USA 81:6851-6855, 1984.
McDougal, et al., "Binding of HTLV-III/LAV to T4+ T Cells by a Complex of the 110K Viral Protein and the T4 Molecule," Science 231:382-385, 1986.
Maddon, et al., "The Isolation and Nucleotide Sequence of a cDNA Encoding the T Cell Surface Protein T4: A New Member of the Immunoglobulin Gene Family," Cell 42:93-104, 1985.
Capon, et al., "Designing CD4 Immunoadhesins for AIDS Therapy," Nature 337:525-531, 1989.
Gascoigne, N.R.J. et al., "Secretion of a Chimeric T-cell Receptor-immunoglobulin protein," Proc. Natl. Acad. Sci. (USA) 84:2936-2940 (1987).
Lasky, L. A. et al., "Delineation of a Region of the Human Immuno-deficiency Virus Type 1 gp120 Glycoprotein Critical for Interaction with the CD4 Receptor," Cell 50:975-985 (1987).
Traunecker, A. et al., "Soluble CD4 Molecules Neutralize Human Immuno-deficiency Virus Type 1," Nature 331:84-86 (1988).
Kowalski, M. et al., "Functional Regions of the Envelope Glycoprotein of Human Immunodeficiency Virus Type I," Science 237:1351-1355 (1987).
Ward et al., "Prevention of HIV-1 ITIB Infection in Chimpanzees by CD4 Immunoadhesin," Nature 352:434-436 (1991).
Putkonen et al., "Prevention of HIV-2 and SIVsm Infection by Passive Immunization in Cynomolgus Monkeys," Nature 352:436-38 (1991).
General Hospital Corporation
Ulm John
LandOfFree
Cloned genes encoding IG-CD4 fusion proteins and the use thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cloned genes encoding IG-CD4 fusion proteins and the use thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cloned genes encoding IG-CD4 fusion proteins and the use thereof will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-94872