Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1989-02-09
1992-06-16
Ellis, Joan
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435 693, 435 91, 4351723, 4353201, 4352351, 43525233, 536 27, 530300, 530350, C12N 121, C12N 1500, C12N 1570, C12P 2102, C12P 1934
Patent
active
051224712
ABSTRACT:
Disclosed are DnA sequences which code for antigenic proteins, methods for identifying such DNA sequences, and antigens coded for by such DNA sequences.
The first step of the method is to provide a multiplicity of DNA sequences. These sequences are then inserted into DNA expression vectors to form recombinant expression vectors. The expression vectors are inserted into suitable hosts to form transformants which express the DNA sequences. The transformants are then contacted with antibodies directed against Eimeria antigens to identify transformants containing DNA sequences which code for Eimeria antigens. These antigens are then produced from the DNA sequences identified as coding for the antigens. The antigens so produced are contacted with white blood cells which effect a cell-mediated immune response, which white blood cells are sensitized to an antigenic Eimeria protein, to thereby identify DNA sequences which code for antigens that induce a cell-mediated immune response to avian coccidiosis.
The DNA sequences of the present invention comprise cloned genes or fragments thereof that code on expression for an antigenic protein that activates white blood cells which effect a cell-mediated immune response, which white blood cells are sensitized to an antigenic Eimeria protein.
REFERENCES:
Livingston et al. Ann Rev Immunol vol. 5 pp. 477-501 (1987).
Lillehoj Vet Immuno Immunopath vol. 13 pp. 321-330 (1986).
Gubler, V. et al. T . Immunol. vol. 136 pp. 2492-2497 (1986).
H. Wisher, "Identification of the Sporozoite Antigens of Eimeria tenella," Molec. Biochem. Parasitol. 21:7-15 (1986).
M. C. Jenkins et al., "Identification of Immunodominant Surfact Antigens of Eimeria acervulina Sporozoites and Merozoites," Molec. Biochem. Parasitol. 25:155-164 (1987).
M. C. Jenkins et al., "Eimeria acervulina: DNA Cloning and Characterization of Recombinant Sporozoite and Merozoite Antigens," Exp. Parasitol. 66:96-107 (1988).
M. C. Jenkins, "A cDNA Encoding a Merozoite Surface Protein of the Protozoan Eimeria acervulina contains Tandem-Repeated Sequences," Nucleic Acids Res. 16(20):9863 (1988).
Dame John B.
Danforth Harry D.
Jenkins Mark C.
Lillehoj Hyun S.
Ruff Michael D.
Ellis Joan
Fado John D.
Ribando Curtis P.
Silverstein M. Howard
The United States of America as represented by the Secretary of
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