Cloned gene encoding acylcoenzyme A: cholesterol acyltransferase

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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435 15, 435193, 435 691, 4353201, 536 232, 935 14, 935 32, 935 70, 935 59, C12N 910, C12N 1554, C12N 1585, C12Q 148

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054847273

ABSTRACT:
This invention pertains to purified, biologically active acyl coenzyme A: cholesterol acyltransferase (ACAT) and to nucleic acid (DNA or RNA) encoding acyl coenzyme A: cholesterol acyltransferase. The nucleic acid, or a fragment thereof, may be ligated with an expression vector and transfected into cells to express acyl coenzyme A: cholesterol acyltransferase activity in intact cells and in cell-free extracts.

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