Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1994-08-19
1998-02-10
Jacobson, Dian C.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4352402, 43525233, 43525411, 4353201, 536 232, C12N 950, C12N 1555, C12N 1563
Patent
active
057168342
ABSTRACT:
Full-length and deletion subclones of cDNAs for Factor C of Carcinoscorpius rotundicauda are provided. These cDNAs have been cloned into .lambda.gt 22 and pGEM 11Zf(+). Further manipulations of the 5' and 3' ends of these cDNAs have been carried out, and these cDNAs have been further subcloned into other expression vectors such as pGEMEX-1, pET 3b, and the yeast shuttle vectors YEpsec 1 and pEMBLyex 4, and pPIC 9 and pHIL D2. Also provided are host cells transformed with expression vectors containing DNA molecules encoding proteins having Factor C-like enzymatic activity, methods of producing such proteins, methods for purifying Factor C zymogens, and methods for protecting Factor C zymogens from autoactivation by Gram negative bacterial endotoxin while the proenzyme is being purified and/or processed from amoebocyte lysates or from recombinant clones, or during storage or subsequent handling. This protection is afforded by the addition of 5-30% Me.sub.2 SO, which reversibly inhibits the Factor C zymogen.
REFERENCES:
patent: 4322217 (1982-03-01), Dikeman
patent: 5082782 (1992-01-01), Gibson
Ho (1983) Microbios Letters 24:81-84.
Ho et al. (1985) Proc. 1st Intl. Cong. Singapore Soc. Microbiol, pp.664-669.
Kim et al. (1987) Singapore Society for Microbiology, 1987 Annual Scientific Meeting, p. 21.
Ding et al. (1988) Cytobios 55:147-154.
Kim et al. (1988) 7th FAOB Symposium, POS-F-01.
Navas et al. (1988) 5 FAOB Congress, MO:19.
Yeo et al. (1989) Second SSM International Congress for Microbiology, BE8.
Navas et al. (1990) Biochemistry International 21(5):805-813.
B. Ho et al. (1993) Biochemistry and Molecular Biology International 29(4):687-694.
Ding et al (1993) Biochimica et Biophysica Acta 1202:149-156.
Ding et al. (1993) Cytobios 75:21-32.
Muta et al. (1991) The Journal of Biological Chemistry 266(10):6554-6561.
Navas III et al., Proc. 5th FAOB Congress, MO:19, pp. 199 (Aug. 13-18, 1988).
Roopashree et al., Microbiology in the Nineties, pp. 380 (1994).
Ding et al., Biochimica et Biophysica Acta, 1202:149-156 (Sep. 1993).
Ding et al., Essays in Zoology, pp. 337-341 (1990).
Ding Jeak Ling
Ho Bow
Jacobson Dian C.
National University of Singapore
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