Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1995-01-09
1999-06-15
Hendricks, Keith D.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
536 232, 4353201, 4352523, 43525233, C12N 912, C12N 1554
Patent
active
059121556
ABSTRACT:
The invention relates to a substantially pure thermostable DNA polymerase from Thermotoga neapolitana (Tne). The Tne DNA polymerase has a molecular weight of about 100 kilodaltons and is more thermostable than Taq DNA polymerase. The present invention also relates to the cloning and expression of the Tne DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes. The Tne DNA polymerase of the invention may be used in well-known DNA sequencing and amplification reactions.
REFERENCES:
patent: H1531 (1996-05-01), Blumentals et al.
patent: 4795699 (1989-01-01), Tarbor et al.
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5047342 (1991-09-01), Chatterjee
patent: 5614365 (1997-03-01), Tabor et al.
patent: 5624833 (1997-04-01), Gelfand et al.
Derbyshire et al., The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction, EMBO J. 10: 17-24 (1990).
Derbyshire et al., Genetic and Crystallographic Studies of the 3', 5'-Exonucleolytic Site of DNA Polymerase I, Science, vol. 240, 199-201 (1988).
Polesky et al., Side Chains Involved in Catalysis ofthe Polymerase Reaction of DNA Polymerase I from Escherichia coli, J. Bio. Chem., vol. 267, No. 12: 8417-8428 (1992).
Polesky et al., Identification of Residues Critical for the Polymerase Activity of the Klenow Fragment of DNA Polymerase I from Escherichia coli, J.Bio. Chem., vol. 265, No. 24;14579-14591 (1990).
D.L. Ollis et al., Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP, Nature, vol. 313: 762-766 (1985).
C.M. Joyce, Can DNA polymerase I (Klenow fragment) serve as a model for other polymerases?, Current Opin.Struct. Bio. vol 1: 123-129 (1991).
Bernad et al., A Conserved 3'-5' Exonuclease Active Site in Prokaryotic and Eukaryotic DNA Polymerases, Cell, vol. 59: 219-228 (1989).
D. Gutman et al., Conserved sites in the 5'-3' exonuclease domain of Escherichia coli DNA polymerase, Nuc. Acids Res., vol. 21, No. 18: 4406-4407 (1993).
D.K. Braithwaite et al., Compilation, alignment, and phylogenetic relationships of DNA polymerases, Nuc. Acids Res., vol. 21, No. 4: 787-802 (1993).
Shengyu et al., Heat-Stable DNA Polymerase I Large Fragment Resolves Hairpin Structure In DNA Sequencing, Scientia Sinica (Series B), vol. XXX; 503-507 (1987).
Jannasch et al., "Thermotoga neapolitana sp. nov. of the Extremely Thermophilic, Eubacterial Genus Thermotoga," Archives of Microbiology 150(1):103-104 (1988).
Slater et al., "DNA Polymerase I of Thermatoga neopolitane (Tne) and Mutant Derivatives," Seventh International Genome Sequencing and Analysis Conference, Sep. 16-20, 1995, Abstract.
Bergquist et al., Genetics and Potential Biotechnological Applications of Thermophilic and Extremely Thermophilic Microorganisms, Biotech. Genet. Eng. Rev. 5:199-244 (1987).
Chien et al., Deoxyribonucleic Acid Polymerase from Extreme Thermophile Thermus aquaticus, J. Bacteriology 127:1550-1557 (1976).
Darzins and Chakrabarty, Cloning of Genes Controlling Alginate Biosynthesis from a Mucoid Cystic Fibrosis Isolate of Pseudomonas aeruginosa, J. Bacteriol. 159:9-18 (1984).
Elie et al., Thermostable DNA polymerase from the archaebacterium Sulfolobus acidocaldarius Eur. J. Biochem. 178:619-626 (1989).
Huber et al., Thermotoga maritima sp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90 degree celsius, Arch. Microbiol. 144:324-333 (1986).
Huser et al., Isolation and characterization of a novel extremely thermophilic, anaerobic, chemo-organothrophic eubacterium, FEMS Microbiology Letters 37:121-127 (1986).
Kaboev et al., Purification and Properties of Deoxyribonucleic Acid Polymerase from Bacillus stearothermophilus, J. Bacteriol. 145(1):21-26 (1981).
Kaledin et al., Isolation and Properties of DNA Polymerase from Extremely Thermophilic Bacterium Thermus aquaticus YT1, Biokhimiya 45:644-51 (1980).
Kelly et al., Extremely Thermophilic Archaebacteria: Biological and Engineering Consideration, Biotechnol. Prog. 4(2):47-62 (1988).
Klimczak et al., Purification and Characterization of DNA Polymerase from the Archaebacterium Methanobacterium thermoautotrophicum, Biochemistry 25:4850-4855 (1986).
Lin et al., Cloning and expressing of T4 DNA polymerase, Proc. Natl. Acad. Sci. USA 84:7000-7004 (1987).
Minkley et al., Escherichia coli DNA Polymerase I, J. Biol. Chem. 259(16):10386-10392 (1984).
Rossi et al., Structure and Properties of a Thermophilic and Thermostable Polymerase from Sulfolobus solfataricus, System. Appl. Microbiol. 7:337-341 (1986).
Sagner et al., Rapid filter assay for the detection of DNA polymerase activity: direct identification of the gene for the DNA polymerase from Thermus aquaticus, Gene 97:119-123 (1991).
Simpson et al., Purification and some properties of a thermostable DNA polymerase from a Thermotoga species, Biochem. Cell Biol. 86:1292-1296 (1990).
Stenesh et al., DNA Polymerase from Mesophilic and Thermophilic Bacteria, I. Purification and properties of DNA polymerase from Bacillus licheniformis and Bacillus stearothermophilus, Biochim. Biochys. Acta 272:156-166 (1972).
Windberger et al., Thermotoga thermarum sp. nov. and Thermotoga neapolitana occurring in African continental solfataric springs, Arch. Microbiol. 151: 506-512 (1989).
Chatterjee Deb K.
Hughes, Jr. A. John
Hendricks Keith D.
Life Technologies Inc.
LandOfFree
Cloned DNA polymerases from Thermotoga neapolitana does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cloned DNA polymerases from Thermotoga neapolitana, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cloned DNA polymerases from Thermotoga neapolitana will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-402040