Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 8 to 10 amino acid residues in defined sequence
Patent
1997-11-11
1999-06-08
Prouty, Rebecca E.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
8 to 10 amino acid residues in defined sequence
435193, C07K 706, C12N 910
Patent
active
059105704
ABSTRACT:
The present invention relates to a method for the isolation and expression of a glycosyltransferase enzyme for use in the synthesis of oligosaccharide or polysaccharide structures on glycoproteins, glycolipids, or as free molecules. The gene coding for the enzyme N-acetylgalactosaminyltransferase and the polypeptide sequence of the acceptor peptide for the enzyme N-acetylgalactosaminyltransferase have been isolated and used for the control of glycosylation of a protein.
REFERENCES:
patent: 4521592 (1985-06-01), Dahmen et al.
patent: 4657849 (1987-04-01), Kallenius et al.
patent: 4665060 (1987-05-01), Mardh et al.
patent: 4762824 (1988-08-01), Kallenius et al.
patent: 4801583 (1989-01-01), Petitou et al.
patent: 4810784 (1989-03-01), Larm
patent: 4851338 (1989-07-01), Mardh et al.
Aubert, J.-P., Biserte, G. and M-H Loucheux-Lefebvre, "Carbohydrate-Peptide Linkage in Glycoproteins," Arch. Biochem. Biophys., 175:410-418 (1976).
Bourdon, M.A., Krusius, T., Campbell, S., Schwartz, N.B., and E. Ruoslahti, "Identification and synthesis of a recognition signal for the attachment of glycosaminoglycans to proteins," Proc. Natl. Acad. Sci. USA, 84:3194-3198 (1987).
Briand, J.P., Andrews, S.P., Jr., Cahill, E., Conway, N.A., and J.D. Young, "Investigation of the Requirements for O-Glycosylation by Bovine Submaxillary Gland UDP-N-Acetylgalactosamine: Polypeptide N-Acetylgalactosamine Transferase Using Synthetic Peptide Substrates," J. Biol. Chem., 256(23):12205-12207 (1981).
Bushway, A. A., and T. W. Keenan, "Characterization of a Soluble Glycolipid Galactosyltransferase Which Occurs in Bovine Milk," Biochim. Biophys. Acta, 572:146-152 (1979).
Carlson, D.M., "Structures and Immunochemical Properties of Oligosaccharides Isolated from Pig Submaxillary Mucins," J. Biol. Chem., 243(3):616-626 (1968).
Chen, W., and O. P. Bahl, "Recombinant Carbohydrate Variant of Human Choriogonadotropin .beta.-Subunit (hCG.beta.) Descarboxyl Terminus (115-145)," J. Biol. Chem., 266(10):6246-6251 (1991).
Chou, P.Y., and G.D. Fasman, "Empirical Predictions of Protein Conformation," Ann. Rev. Biochem., 47:251-276 (1978).
Cruz, T.F., and M.A. Moscarello, "Identification of the Major Sites of Enzymic Glycosylation of Myelin Basic Protein," Biochim. Biophys. Acta, 760:403-410 (1983).
Davis, C. G., Elhammer, A., Russell, D.W., Schneider, W.J., Kornfeld, S., Brown, M.S., and J.L. Goldstein, "Deletion of Clustered O-Linked Carbohydrates Does Not Impair Function of Low Density Lipoprotein Receptor in Transfected Fibroblasts," J. Biol. Chem., 261(6):2828-2838 (1986).
Deschuyteneer, M., Eckhardt, A.E., Roth, J. and R.L. Hill, "The Subcellular Localization of Apomucin and Nonreducing Terminal N-Acetylgalactosamine in Porcine Submaxillary Glands," J. Biol. Chem., 263(5):2452-2459 (1988).
Devereux, J., Haeberli, P. and O. Smithies, "A comprehensive set of sequence analysis programs for the VAX," Nucleic Acids Res., 12(1):387-395 (1984).
Domingo, D. L., and I. S. Trowbridge, "Characterization of the Human Transferrin Receptor Produced in a Baculovirus Expression System," J. Biol. Chem., 263(26):13386-13392 (1988).
Dunphy, W. G., Brands, R., and J.E. Rothman, "Attachment of Terminal N-Acetylglucosamine to Asparagine-Linked Oligosaccharides Occurs in Central Cisternae of the Golgi Stack," Cell, 40:463-472 (1985).
Eisenberg, D., "Three-Dimensional Structure of Membrane and Surface Proteins," Ann. Rev. Biochem., 53:595-623 (1984).
Elhammer, A.P. et al., "The Specificity of UDP-GalNAc: Polypeptide N-Acetylgalactosaminyltransferase as Inferred from a Database of in Vivo Substrates and from the in Vitro Glycosylation of Proteins and Peptides," J. of Biological Chemistry, 268(14):10029-10038 (1993).
Elhammer, A.P. & S. Kornfeld,"Purification and Characterization of UDP-N-Acetylgalactosamine: Polypeptide N-Acetylgalactosaminlytransferase from Bovine Colostrum and Murine Lymphoma BW5147 Cells," J. of Biological Chemistry, 261(12):5249-5255 (1986).
Elhammer, .ANG..P., and S. Kornfeld, "Two Enzymes Involved in the Synthesis of O-linked Oligosaccharides are Localized on Membranes of Different Densities in Mouse Lymphoma BW5147 Cells," J. Cell Biol., 98:327-331 (1984).
Fraser, M.J., The Baculovirus-Infected Insect Cell as a Eukaryotic Gene Expression System, Current Topics Microbiol. Immunol., 158:131-172 (1992).
Garnier, J., Osguthorpe, D.J., and B. Robson, "Analysis of the Accuracy and Implications of Simple Methods for Predicting the Secondary Structure of Globular Proteins," J. Mol. Biol., 120:97-120 (1978).
Goldin, A.L., Sandri-Goldin, R.M., Levine, M., and J.C. Glorioso, "Cloning of Herpes Simplex Virus Type 1 Sequences Representing the Whole Genome," J. Virol., 38(1):50-58 (1981).
Gooley, A.A., Classon, B.J., Marschalek, R., and K.L. Williams, "Glycosylation Sites Identified by Detection of Glycosylated Amino Acids Released From Edman Degradation: The Identification of Xaa-Pro-Xaa-Xaa as a Motif for Thr-O-Glycosylation," Biochem. Biophys. Res. Commun., 178(3):1194-1201 (1991).
Hagopian, A., and E.H. Eylar, "Glycoprotein Biosynthesis: Studies on the Receptor Specificity of the Polypeptidyl: N-Acetylgalactosaminyl Tranferase from Bovine Submaxillary Glands," Arch. Biochem. Biophys., 128:422-433 (1968).
Hagopian, A., Westall, F.C., Whitehead, J.S., and E.H. Eylar, "Glycosylation of the A1 Protein from Myelin by a Polypeptide N-Acetylgalactosaminyltransferase," J. Biol. Chem., 246(8):2519-2523 (1971).
Haltiwanger, R.S., Holt, G.D. and G.W. Hart, "Enzymatic Addition of O-GlcNAc to Nuclear and Cytoplasmic Proteins," J. Biol. Chem., 265(5):2563-2568 (1990).
Hanover, J.A., Elting, J., Mintz, G.R. and W.J. Lennarz, J. Biol. Chem., 257(17):10172-10177 (1982).
Hardy, M.R., Townsend, R.R. and Y.C. Lee, "Monosaccharide Analysis of Glycoconjugates by Anion Exchange Chromatography with Pulsed Amperometric Detection," Anal. Biochem., 170:54-62 (1988).
Hart, G.W., Holt, G.D. and R.S. Haltiwanger, "Nuclear and cytoplasmic glycosylation: novel saccharide linkages in unexpected places," TIBS, 13:380-384 (1988).
Heinrikson, R.L., Sterner, R. and C. Noyes, "On the Subunit Structure of Yeast Inorganic Pyrophosphatase," J. Biol. Chem., 248(7):2521-2528 (1973).
Hill, Jr., H.D., Schwyzer, M., Steinman, H.M. and R.L. Hill, "Ovine Submaxillary Mucin," J. Biol. Chem., 252(11):3799-3804 (1977).
Homa, F.L. et al., "Isolation and Expression of a cDNA Clone Endcoding a Bovine UDP-GalNAc: Polypeptide N-Acetylgalactosaminyltransferase," J. of Biological Chemistry, 268(17):12609-12616 (1993).
Database Embl.--Homa, F.L. et al., "Isolation and expression of cDNA clone encoding a bovine UDP-GalNAc: polypeptide, N-acetylgalactosaminyltransferase," AN:L07780, May 7, 1993.
Homa, F. L., Otal, T.M., Glorioso, J.C. and M. Levine, "Transcriptional Control Signals of a Herpes Simplex Virus Type 1 Late (.gamma.2) Gene Lie within Bases -34 to +124 Relative to 5' Terminus of the mRNA," Mol. Cell. Biol., 6(11):3652-3666 (1986).
Hsieh, P., and P.W. Robbins, "Regulation of Asparagine-linked Oligosaccharide Processing," J. Biol. Chem., 259(4):2375-2382 (1984).
Hughes, R.C., Bradbury, A.F. and D.G. Smyth, "Substrate Recognition by UDP-N-Acetyl-.alpha.-D-Galactosamine: Polypeptide N-Acetyl-.alpha.-D-Galactosaminyltransferase. Effects of Chain Length and Disulphide Bonding of Synthetic Peptide Substrates," Carbohydr. Res., 178:259-269 (1988).
Jensenius, J.-C., Andersen, I., Hau, J., Crone, M. and C. Koch, "Eggs: Conveniently Packaged Antibodies. Methods for Purification of Yolk IgG," J. Immunol. Meth., 46:63-68 (1981).
Jentoft, N., "Why are proteins O-glycosylated?" Trends Biochem. Sci., 15:291-294 (1990).
Joziasse, D. H., "Mammalian glycosyltransferases: genomic organization and protein structure," Glycobiology, 2:271-277 (1992).
Kabsch, W., and C. Sander, "Dictionary of Protein Secondary Structure: Pattern Recognition of Hydrogen-Bonded and Geometrical Features," Biopolymers, 22:2577-2637 (1983).
Kaushansky, K., Lopez, J.A. and C.B. Brown, "Role of Carbohydrate Modification in the Production and Secretion of Human Granulocyte Macrophage Colony-Stimulating Factor in Genetically Engineer
Elhammer Ake P.
Homa Fred L.
Darnley, Jr. James D.
Pharmacia & Upjohn Company
Prouty Rebecca E.
LandOfFree
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