Cloned arabidopsis p-hydroxyphenyl pyruvic acid dioxygenase DNA

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

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435243, 4352523, 4353201, 435419, 536 232, 536 236, 800278, 800298, A01H 500, A01H 510, C12N 121, C12N 514, C12N 1529, C12N 1552, C12N 1582

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060875631

ABSTRACT:
A cDNA clone from Arabidopsis thaliana, pHPP1.5, SEQ ID NO:1, which encodes the enzyme p-hydroxyphenyl pyruvic acid dioxygenase, is disclosed. A vector and microbial host containing a DNA sequence coding for the expression of Arabidopsis thaliana p-hydroxyphenyl pyruvic acid dioxygenase, and a genetic construct containing a DNA sequence coding for the expression of Arabidopsis thaliana p-hydroxyphenyl pyruvic acid dioxygenase, together with a promoter located 5' to the DNA coding sequence and a 3' termination sequence, are also disclosed. A method of creating a transgenic plant comprising said genetic construct is also disclosed.

REFERENCES:
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Endo et al. Primary structure deduced from complementary DNA sequence and expression in cultured cells of mammalian 4-hygroxyphenylpyruvic acid dioxygenase. The Journal of Biological Chemistry. 267(34):24235-24240, 1992.
Newman et al. Genes galore: a summary of methods for accessing results from large-scale partial sequencing of anonymous Arabidopsis cDNA clones. Plant Physiology. 106:1241-1255, 1994.
MPSEARCH results 1 and 2 of SEQ ID No:1: Locus N65764, Accession No. N65764. Newman et al., as above, 1994.
MPSEARCH Result 2 of SEQ ID NO:2: Locus PIGHPD, Accession No. D13390. Endo et al., as above, 1992.
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Ruetschi, Ulla, et al., "Characterization of 4-hydroxyphenylpyruvate dioxygenase: Primary structure of the Pseudomonas enzyme," Eur. J. Biochem., 205:459-466 (1992).
Schultz, Arno, et al., "SC-0051, a 2-benzoyl-cyclohexane-1,3-dione bleaching herbicide, is a potent inhibitor of the enzyme p-hydroxyphenylpyruvate dioxygenase," FEBS 12117318:162-166 (1993).
Secor, Jacob, "Inhibition of Barnyardgrass 4-hydroxyphenylpyruvate Dioxygenase by Sulcotrione," Plant Physiol. 106:1429-1433 (1994).
Soeda, Takashi, et al., "Inhibition of Pigment Synthesis by 1,3-Dimethyl-4-(2,4-dichlorobenzoyl)-5-hydroxypyrazole, Norflurazon, and New Herbicidal Compounds in Radish and Flatsedge Plants," Pesticide Biochemistry and Physiology 29:35-42 (1987).
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