Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-04-28
1998-06-09
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435201, 435210, 435 691, 4353201, 4352523, 536 232, C12N 924, C12N 944, C12N 120, C07H 2104
Patent
active
057632529
ABSTRACT:
A cDNA clone from barley, pAGL.2737, SEQ ID NO: 3, which encodes the enzyme .alpha.-glucosidase, is disclosed. A vector and microbial host containing a DNA sequence coding for the expression of barley .alpha.-glucosidase, and a DNA construct comprising a DNA sequence coding for the expression of barley .alpha.-glucosidase, together with a promoter located 5' to the DNA coding sequence and a 3' termination sequence, are also disclosed.
REFERENCES:
Tibbot et al (1996) Plant Mol. Biol. 30:229-241. "Molecular Cloning and Characterization of a Giberellin-Inducible, Putative .gamma.-Glucosidase From Barley".
Henson, C.A., and R.W. Skadsen, "Improved Starch Hydrolysis Using Barley Alpha-Gucosidase," Grant Application to Midwest Plant Biotechnology Consortium (1992).
Hoefsloot, L.H., et al., "Primary Structure and Processing of Lysosomal .alpha.-glucosidase: Homology with the Intestinal Sucrase-Isomaltase Complex," The EMBO J., 7:1697-1704 (1988).
Kinsella, B.T., et al., "Primary Structure and Processing of the Candida tsukubaensis .alpha.-glucosidase: Homology with the Rabbit Intestinal Sucrase-Isomaltase Complex and Human Lysosomal .alpha.-glucosidase," FEBS, (1991). Eur J Biochem 202:657-664.
Martiniuk, F., et al., "Sequence of the cDNA and 5'-Flanking Region for Human Acid .alpha.-Glucosidase, Detection of an Intron in the 5' Untranslated Leader Sequence, Definition of 18-bp Polymorphisms, and Differences with Previous cDNA and Amino Acid Sequences," DNA and Cell Biol., 9:85-94 (1990).
Naim, H.Y., et al., "Striking Structural and Functional Similarities Suggest that Intestinal Sucrase-Isomaltase, Human Lysosomal .alpha.-Glucosidase and Schwanniomyces occidentalis Glucoamylase are Derived from a Common Ancestral Gene," FEBS 294:100-112 (1991).
Newman, T., "380 Arabiodopsis thaliana cDNA Clone 38A2T7," DNA Sequence Accession No. T04333, Unpublished (1993).
Sun, Z., and C.A. Henson, "Degradation of Native Starch Granules by Barley .alpha.-Glucosidases," Plant Physiol., 94:320-327 (1990).
Sun, Z., and C.A. Henson, "A Quantitative Assessment of the Importance of Barley Seed .alpha.-Amylase, .gamma.-Amylase, Debranching Enzyme, and .alpha.-Glucosidase in Starch Degradation," Arch. Biochem. Biophys., 284:298-305 (1991).
Svensson, B., "Regional Distant Sequence Homology Between Amylases, .alpha.-Glucosidases and Transglucanosylases," FEBS Letters, 230:72-76 (1988).
Tibbot, B.K. and R.W. Skadsen, "Cloning of A GA.sub.3 -Responsive Alpha-Glucosidase cDNA From the Aleurone of Germinating Barley and Analysis of Its Expression," Supplement to Plant Physiology, 105 (1994).
Skadsen Ronald W.
Tibbot Brian K.
Mytelka Daniel
Wax Robert A.
Wisconsin Alumni Research Foundation
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