Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1985-02-11
1987-05-12
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435241, 47 58, C12N 500, A01B 7900
Patent
active
046650318
ABSTRACT:
A method for the vegetative propagation of the sea kale species is described. Buds are obtained from in vitro neoformation on a callus, which in turn is obtained from fragments of petioles or leaf laminas of the plant. The rootless plants are then micropropogated in vitro in a plant culture medium and cytokinines in the absence of auxins. The rootless plants bearing one or two leaves are cultured in vitro in a plant growth broth and one or more auxins in the absence of cytokinins until roots develop. The rooted plants are transferred to a solid plant growth substrate and bred under conditions of a natural photoperiod of illumination until a plant is obtained which is capable of growth in the field.
REFERENCES:
patent: 4038778 (1977-08-01), Kadkade
patent: 4569914 (1986-02-01), Molnar et al.
Flick et al. 1983 "Organogenesis" in Handbook of Plant Cell Culture, vol. 1, (Evans et al., eds.) pp. 13, 27, 28.
Bowes, 1976, "In vitro Morphogenesis of Crambe maritima L." Protoplasma, v89, 185-188 (Abstract).
Ecole Nationale d'Ingenieurs des Travaux
Shapiro Lionel M.
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