Clipsin, a chymotrypsin-like protease and method of using same

Details Clipsin, a chymotrypsin-like protease and method of using same Clipsin, a chymotrypsin-like protease and method of using same

1990-06-22

1993-10-12

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435184, 435212, 424 9464, C12Q 137

Patent

active

052524639

ABSTRACT:
A novel M.sub.r 25,000 chymotrypsin-like protease, termed clipsin, which binds to a-1-AChy in a sodium dodecyl sulfate-resistant manner, preferentially degrades .beta.-APP, and shows high activity for a period of a few days in neonatal rat brain is disclosed. Preparation of clipsin in a form that allows clipsin proteolytic activity to be measured under conditions substantially free of the activity other cellular non-clipsin proteases is also disclosed. Further disclosed is a method of identifying potential therapeutic agents for the treatment of selected neuropathologies such as Down's syndrome and Alzheimer's disease by measuring or inhibition of clipsin.

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