Clinical determination and/or quantification of thyrotropin and

Details Clinical determination and/or quantification of thyrotropin and Clinical determination and/or quantification of thyrotropin and

1983-05-31

1986-09-02

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

436500, 436506, 435 4, 435 35, 435240, 435948, G01N 3353, C12Q 102, C12N 500

Patent

active

046096221

ABSTRACT:
A fast growing, continuous, functional rat thyroid cell strain, FRTL-5, which maintains functional characteristics of iodide uptake and thyroglobulin synthesis over prolonged periods of culture is cloned from FRTL cells obtained from primary cultures of Fischer rat thyroid glands. The FRTL-5 cells are cultured in a medium containing approximately 5 percent calf serum supplemented with a mixture of hormones, at least one of which is thyrotropin.
The FRTL-5 cells are employed in a series of assays which measure thyroid stimulatory or inhibitory factors. The FRTL-5 system of assay specifically measures thymidine incorporation, cAMP elevation and iodide uptake and permits the evaluation of patient sera, particularly those afflicted with Graves' disease and other autoimmune thyroid diseases, thereby providing a means for determining appropriate methods of treatment.

REFERENCES:
Beguinot, F. et al, Biochem. Biophys. Res. Commun., vol. 110, pp. 48-54 (1-1983).
Vihi, P. et al, J. Clin. Endocr. Metab., vol. 57(4), pp. 782-791 (1983).
Valente, W. A. et al, Endocrinology, vol. 112(1), pp. 71-79 (1983).
Ambesi-Impiombato, F. S. et al, Cold Spring Harbor Conf., vol. 9, pp. 483-492 (1982).
Ambesi-Impiambato, F. S. et al, Cold Spring Harbor Conf., vol. 9, p. 141ff, (1982).
Kasagi, K. et al, J. Clin. Endocr. Metab., vol. 54, pp. 108-114 (1982).
Kohn, L. D. et al, (Dev. Cancer Research, vol. 9), Membranes in Tumor Growth, Galeotti et al, ed, Elsevier Press (1982), pp. 389-395.
Tramontano, D. et al, Exper. Cell Research, vol. 137, pp. 269-275 (1982).
Vitti, P. et al, J. Endocrinol. Invest., vol. 5, pp. 179-182 (1982).
Avivi, A. et al, Science, vol. 214, pp. 1237-1239 (1981).
DeWolf, M. J. S. et al, J. Biol. Chem., vol. 256(23), pp. 12287-12296 (1981).
Magnvsson, R. P., Fed. Proc., vol. 40(6), p. 1785, abstract 1410 (1981).
Mandato, E. et al, Biochimica et Biphysica Acta, vol. 676, pp. 91-100 (1981).
Ambesi-Impiambato, F. S. et al, Proc. Natl. Acad. Sci., U.S.A., vol. 77(6), pp. 3455-3459 (6-1980).
Ambesi-Impiambato, F. S. et al, Int. Rev. Cytol., Suppl. 10, pp. 163-172 (1979).
Westermark, B. et al, Proc. Natl. Acad. Sci., U.S.A., vol. 76(4), pp. 2022-2026 (4-1979).
Meldolesi, M. F. et al, Ann. Endocrinol., vol. 39(5), p. 28A, Abstract 50(1978).
Mandato, E. et al, Ann. Endocrinol., vol. 38(5), p. 64A, abstract 112 (1977).
Lissitzlcy, S. et al, Evr. J. Biochem., vol. 24, pp. 88-99 (1971).
Winand; R. J. et al, J. Biol. Chem., vol. 250(16), pp. 6534-6540 (1975).
Fayet, G. et al, Molecular and Cellular Endocrin., vol. 7, pp. 67-78 (1977).
Dickson, J. G. et al, J. Endocrin., vol. 90, pp. 113-124 (1981).
Filetti, S. et al, Endocrinology, vol. 113(5), pp. 1608-1615 (1983).
Filetti, S. et al, Endocrinology, vol. 114(4), pp. 1379-1384 (1984).
Rotella, C. M. et al, J. Receptor Research, vol. 2, pp. 367-382 (1982).
Bachrach, L. K. et al, Endocrine Research and Therapy, vol. 2, pp. 263-270 (1985).
Eggo, M. C. et al, Endocrine Research and Therapy, vol. 2, pp. 201-210 (1985).

Affiliated with

Also associated with

Rating

Clinical determination and/or quantification of thyrotropin and does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Clinical determination and/or quantification of thyrotropin and , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Clinical determination and/or quantification of thyrotropin and will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1097530