Cleavage of precursors of insulins by a variant of trypsin

Details Cleavage of precursors of insulins by a variant of trypsin Cleavage of precursors of insulins by a variant of trypsin

2011-07-19

2011-07-19

Nashed, Nashaat (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

C435S213000

Reexamination Certificate

active

07981635

ABSTRACT:
The present invention relates to the production of a variant of recombinant trypsin with increased substrate specificity for arginine versus lysine in non-animal host organisms. Moreover, the present invention relates to a variant of recombinant trypsin and their production. Also provided are use of recombinant porcine pancreatic trypsin variants for cleavage of precursors of insulins, and kits containing the variant of trypsin.

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