Cleavage of nucleic acids

Details Cleavage of nucleic acids Cleavage of nucleic acids

2005-08-05

2009-10-13

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C424S094100

Reexamination Certificate

active

07601496

ABSTRACT:
The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.

REFERENCES:
patent: 4511502 (1985-04-01), Builder et al.
patent: 4511503 (1985-04-01), Olson et al.
patent: 4512922 (1985-04-01), Jones et al.
patent: 4518526 (1985-05-01), Olson
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 5108892 (1992-04-01), Burke et al.
patent: 5144019 (1992-09-01), Rossi et al.
patent: 5310652 (1994-05-01), Gelfand et al.
patent: 5422242 (1995-06-01), Young
patent: 5422253 (1995-06-01), Dahlberg et al.
patent: 5455170 (1995-10-01), Abramson et al.
patent: 5501963 (1996-03-01), Burckhardt
patent: 5541311 (1996-07-01), Dahlberg et al.
patent: 5614402 (1997-03-01), Dahlberg et al.
patent: 5874283 (1999-02-01), Harrington et al.
patent: 6528254 (2003-03-01), Sorge
patent: 0 482 714 (1992-04-01), None
patent: WO 90/01069 (1990-02-01), None
patent: WO 90/03443 (1990-05-01), None
patent: WO 90/15157 (1990-12-01), None
patent: WO 91/09950 (1991-07-01), None
patent: WO 92/06200 (1992-04-01), None
Moradpour et al. Hepatitis C: an update. Swiss Med Wkly. Jun. 2, 2001;131(21-22):291-8.
Watson et al. Molecular Biology of the Gene, Fourth Edition, vol. 1, Benjamin/Cummings Publishing Co., Inc. CA, 1987, pp. 245-246.
Cariello et al. (1991) Nucl. Acids Res. 19:4198.
Lundberg et al. (1991) Gene 108:1-6.
Harrington et al. (1994) Genes and Develop. 8:1344-1355.
Shen et al. (1996) J. Biol. Chem. 271:9173-9176.
Shen et al. (1997) Nucleic Acids Res. 25:3332-333.
Barany, “Genetic disease detection and DNA amplification using cloned thermostable ligase,”Proc. Natl. Acad. Sci., 88:189 (1991).
Barany, “The Ligase Chain Reaction in a PCR World,”PCR Methods and Applic., 1:5 (1991).
Wu and Wallace, “The Ligation Amplification Reaction (LAR)—Amplification of Specific DNA Sequences Using Sequential Rounds of Template-Dependent Ligation,”Genomics4:560 (1989).
Guatelli et al., “Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication,” Proc. Natl. Acad. Sci., 87:1874-1878 (1990) with an erratum at Proc. Natl. Acad. Sci., 87:7797 (1990).
Kwoh et al., “Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format,”Proc. Natl. Acad. Sci., 86:1173-1177 (1989).
Fahy et al., “Self-sustained Sequence Replication (3SR): An Isothermal Transcription-based Amplification System Alternative to PCR,”PCR Meth. Appl., 1:25-33 (1991).
Landgren, “Molecular mechanics of nucleic acid sequence amplification,”Trends in Genetics9:199 (1993).
Mullis, “The Polymerase Chain Reaction in an Anemic Mode: How to Avoid Cold Oligodeoxyribonuclear Fusion,”PCR Methods Applic., 1:1 (1991).
Kwok et al., “Effects of primer-template mismatches on the polymerase chain reaction: Human immunodeficiency virus type 1 model studies,”Nucl. Acids Res., 18:999 (1990).
Duck et al., “Probe Amplifier System Based on Chimeric Cycling Oligonucleotides,”BioTech., 9:142 (1990).
Urdea et al., “A novel method for the rapid detection of specific nucleotide sequences in crude biological samples without blotting or radioactivity; application to the analysis if hepatitis B virus in human serum,”Gene61:253-264 (1987).
Gogos et al., “Detection of single base mismatches of thymine and cytosine residues by potassium permanganate and hydroxylamine in the presence of tetralkylammonium salts,”Nucl. Acids. Res., 18:6807-6817 (1990).
Barlow and Lehrach, “Genetics by gel electrophoresis: the impact of pulsed field gel electrophoresis on mammalian genetics,”Trends Genet., 3:167 (1987).
Perlman and Butow, “Mobile Introns and Intron-Encoded Proteins,”Science246:1106 (1989).
Conner, et al, “Detection of sickle cell βg-globin allele by hybridization with synthetic oligonucleotides,”Proc. Natl. Acad. Sci. 80:278-282 (1983).
Vogelstein et al., “Genetic Alterations During Colorectal-Tumor Development,”N. Eng. J. Med. 319:525-532 (1988).
Farr et al., “Analysis ofRASgene mutations in acute myeloid leukemia by polymerase chain reaction and oligonucleotide probes,”Proc. Natl. Acad. Sci. 85:1629-1633 (1988).
Lyons, et al., “Two G Protein Oncogenes in Human Endocrine Tumors,”Science249:655-659 (1990).
Abrams et al., “Comprehensive Detection of Single Base Changes in Human Genomic DNA Using Denaturing Gradient Gel Electrophoresis and a GC Clamp,”Genomics7:463-475 (1990).
Sheffield, et al., “Attachment of a 40-base-pair G+C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes,”Proc. Natl. Acad. Sci.86:232-236 (1989).
Lerman and Silverstein, “Computational Simulation of DNA Melting and Its Application to Denaturing Gradient Gel Electrophoresis,”Meth. Enzymol., 155:482-501 (1987).
Wartell et al., “Detecting base pari substitutions in DNA fragments by temperature-gradient gel electrophoresis,”Nucl. Acids Res., 18:2699-2701 (1990).
Smith et al., “Novel Method of Detecting Single Base Substitutions in RNA Molecules by Differential Melting Behavior in Solution,”Genomics3:217-223 (1988).
Borresen et al., “Constant denaturant gel electrophoresis as a rapid screening technique for p53 mutations,”Proc. Natl. Acad. Sci. USA88:8405 (1991).
Scholz, et al., “Rapid screening for Tp53 mutations by temperature gradient gel electrophoresis: a comparison with SSCP analysis,”Hum. Mol Genet.2:2155 (1993).
Hayashi, “PCR-SSCP: A Simple and Sensitive Method for Detection of Mutations in the Genomic DNA,”PCR Meth. Appl., 1:34-38, (1991).
Orita, et al., “Rapid and Sensitive Detection of Point Mutations and DNA Polymorphisms Using the polymerase Chain Reaction,”Genomics5:874-879, (1989).
Liu and Sommer, “Parameters Affecting the Sensitivity of Dideoxy Fingerprinting and SSCP,”PCR Methods Appl., 4:97 (1994).
Marmur and Lane, “Strand Separation and Specific Recombination in Deoxyribonucleic acids: Biological Studies,”Proc. Natl. Acad. Sci. USA46:453 (1960).
Doty et al., “Strand Separation and Specific Recombination in Deoxyribonucleic Acids: Physical Chemical Studies,”Proc. Natl. Acad. Sci. USA46:461 (1960).
Wallace et al, “Application of synthetic oligonucleotides to the diagnosis of human genetic diseases,”Biochimie67:755 (1985).
Studencki and Wallace, “Allele-Specific Hybridization using Oligonuleotide Probes of Very High Specific Activity: Discrimination of the Human βA-and β9-Globin Genes,”DNA3:1 (1984).
Studencki et al., “Discrimination among the Human βA, βS, βC-Globin Genes Using Allele-Specific Oligonucleotide Hybridization Probes,”Human Genetics37:42 (1985).
Harrington and Lieber, “Functional domains within FEN-1 and RAD2 define a family of structure-specific endonucleases: implications for nucleotide excision repair,”Genes and Develop. 8:1344 (1994).
Murante et al., “The Calf 5′-to 3′-Exonuclease Is Also an Endonuclease with Both Activities Dependent on Primers Annealed Upstream of the Point of Cleavage,”J. Biol. Chem. 269:1191 (1994).
Kornberg,DNA Replication, W.H. Freeman and Co., San Francisco, pp. 127-139 (1980).
Tindall and Kunkell, Fidelity of DNA Synthesis by theThermus aquaticusDNA Polymerase,Biochem. 27:6008 (1988).
Brutlag et al., “An Active Fragment of DNA Polymerase Produced by Proteolytic Cleavage,

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