Cleavage of nucleic acid acid using thermostable methoanococcus

Details Cleavage of nucleic acid acid using thermostable methoanococcus Cleavage of nucleic acid acid using thermostable methoanococcus

1996-11-29

1998-12-01

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 435 18, 435 9153, 435183, 435194, 435195, 435196, 435810, 435822, 436 94, 530350, C12Q 168, C12P 1934, C12N 912, C12N 100

Patent

active

058436693

ABSTRACT:
The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Structure-specific nucleases, including 5' nucleases, thermostable FEN-1 endonucleases and 3' exonucleases, are used to detect and identify target nucleic acids. Methods are provided which allow for the detection specific nucleic acid sequences; these methods permit the detection and identification of mutant and wild-type forms of genes (e.g., human genes) as well as permit the detection and identification of bacterial and viral pathogens in a sample.

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