Cleavage method for acyl thiohydantoins and use of the method in

Details Cleavage method for acyl thiohydantoins and use of the method in Cleavage method for acyl thiohydantoins and use of the method in

1991-10-15

1993-02-09

Housel, James C.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436161, 530345, 530402, 530412, G01N 3368

Patent

active

051852667

ABSTRACT:
Disclosed is a method for enhancing the cleavage of an acyl thiohydantoin bond, for example, for use in C-terminal peptide sequencing. An acyl thiohydantoin is alkylated to form an adduct on the thiohydantoin, and the adduct-containing thiohydantoin is cleaved at its acyl bond by reaction with a cleaving agent under substantially anhydrous, acidic conditions. In C-terminal amino acid sequencing, the cleaved product is analyzed to identify the C-terminal amino acid.

REFERENCES:
patent: 4837165 (1989-06-01), Hawke
patent: 5041388 (1991-08-01), Boyd et al.
patent: 5049507 (1991-09-01), Hawke et al.
patent: 5059540 (1991-10-01), Bailey
Laursen, R. A., et al., "Solid-Phase Methods in Protein Sequence Analysis" in Methods of Biochemical Analysis 26:201-284 (1980).
Miller, C. G., et al., in "Techniques in Protein Chemistry", (Hugh, T. E., ed.), Academic Press, pp. 67-78 (1989).
Miller, C. G., et al., Abstract T188 from the Third Symposium of the Protein Society, Seattle, Wash. (Jul. 29-Aug. 2, 1989).
Schlack, P., et al., Hoppes Seylers Z. Physical Chemistry, 154:126-171 (1926).
Edman, P., Acta Chem. Scand., 4:283-294, 277-282 (1950).
Edman, P., Acta Chem. Scand., 10:761-768 (1956).
Boyd, V. L., et al., Tetrahedron Letters 31:3849-3852 (1990).
Hendrickson et al., Organic Chemistry, 3rd Ed., pp. 697-738, McGraw-Hill, New York, New York (1970).
Inglis, A. S., Analytical Biochemistry, 195:183-196 (1991).

Affiliated with

Also associated with

Rating

Cleavage method for acyl thiohydantoins and use of the method in does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cleavage method for acyl thiohydantoins and use of the method in, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cleavage method for acyl thiohydantoins and use of the method in will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-325172