Cleavable labels for use in binding assays

Details Cleavable labels for use in binding assays Cleavable labels for use in binding assays

1986-04-03

1988-10-25

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

435 7, 436536, 436537, 436538, 436541, 436542, 436545, 436800, 436824, G01N 33543, G01N 33536

Patent

active

047804211

ABSTRACT:
An improvement in an assay method which relies on the detection of a labeled, solubilized specific binding complex is achieved by linking the label to a component of the complex through a bond cleavable under conditions compatible with the assay and with the complex in solution.

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Chromy et al., "Use of N-Methyl-D-glucamine as Buffer in the Determination of Serum Alkaline Phosphotase Activity" Clin. Chem. 27:1729-1732 (1981).
Chu et al., "Synthesis of an Amplifiable Reporter RNA for Bioassays" Nucleic Acids Res. 14:5591-5603 (Jul. 1986).

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