Class II mobilizable gram-negative plasmid

Details Class II mobilizable gram-negative plasmid Class II mobilizable gram-negative plasmid

1983-07-01

1987-07-14

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435253, 435317, 935 26, 935 29, 935 23, 935 72, 536 27, C12N 1500, C12N 120, C12N 100, C07N 1700

Patent

active

046802649

ABSTRACT:
Vectors capable of replication in a broad host range of gram-negative bacteria are provided. The vectors are composite plasmids comprising a segment derived from an E. coli vector plasmid and a segment derived from a broad host range plasmid. The broad host range plasmid segment includes Mob-functions and DNA coding for replication functions with regions non-essential for broad host range replication or mobilizablity having been deleted. Bacterial strains containing the above plasmids as well as methods of cloning DNA employing such plasmids are also provided.

REFERENCES:
Simon et al, Molecular Genetics of the Bacteria-Plant Interaction, (A. Puhler, ed.) pp. 98-106, 1983.
Change et al, J. Bact., vol. 134, pp. 1141-1156, 1978.
8th North American Rhizobium Conference Abstract Book, pp. 1-3, 7-15, 20, 22, 25, 28-29, 42-43, 100-103, 105, 128, 130, 132 and 136 (Aug. 3-7, 1981).
Proceedings, 8th North American Rhizobium Conference, pp. 90-114, (K. W. Clark & J. H. G. Stephens, eds., 1983).
Bagdasarian, M. et al., (1979) in "Plasmids of Medical, Environmental, and Commercial Importance" (K. N. Timmis and A. Puhler, eds.) Elsevier, Neth., Holland.
Bagdasarian, M. et al., (1981) Gene 16: 237-242.
Bagdasarian, M. et al., (1982) Microbial Drug Resistance, Proc. 3rd Int'l. Symp., Tokyo, (S. Mitshuhashi, ed.) p. 183.
Ditta, G. et al., (1980) Proc. Nat. Acad. Sci., U.S.A., 77:7347.
Priefer, U. B. et al., (1980) in "Antibiotic Resistance, Transposition and Other Mechanisms" (S. Mitshuhashi, L. Rosival, V. Krcmery, eds.) Springer-Verlag, pp. 91-95.
Simon, R., (1980) in "Antibiotic Resistance, Transposition and Other Mechanisms" (S. Mitshuhashi, L. Rosival, V. Krcmery, eds.) Springer-Verlag, Berlin, pp. 35-42.
Simon, R. et al., (1983) Analysis of Plasmid Borne Genes in Rhizobium meliloti, by Tn5 Mutagenesis, Winnipeg Symposium, Proc. N. Amer. Rhizobium Conf., Jan. 1983, (meeting held in 1981).

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