Class I-type lysyl-TRNA synthetase

Details Class I-type lysyl-TRNA synthetase Class I-type lysyl-TRNA synthetase

2000-03-10

2002-12-10

Achutamurthy, Ponnathapu (Department: 1652)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

C435S183000

Reexamination Certificate

active

06492131

ABSTRACT:

TECHNICAL FIELD OF THE INVENTION
This invention relates to a new class I-type lysyl-tRNA synthetase isolated from archaebacteria and Borrelia.
BACKGROUND OF THE INVENTION
Lysyl-tRNA synthetase (LysRS) is essential for the translation of lysine codons during protein synthesis. In spite of the necessity for this enzyme in all organisms and the high degree of conservation among aminoacyl-tRNA synthetases (1), genes encoding a LysRS homologue have not been found by sequence similarity searches in the genomes of two Archaea,
Methanococcus jannaschii
(2) and
Methanobacterium thermoautotrophicum
(3). This raises the possibility that. LysRS, like the asparaginyl- and glutaminyl-tRNA synthetases (4), is not present, with lysyl-tRNA (Lys-tRNA) synthesized by tRNA-dependent transformation of a misacylated tRNA (5). Alternatively, these organisms may contain a LysRS activity encoded by a gene sufficiently different to those previously identified to prevent its detection by sequence similarity searches.
SUMMARY OF THE INVENTION
This invention confirms the latter hypothesis and provides isolated and purified class I-type lysyl-tRNA synthetase (hereafter sometimes denoted herein as LysRSI) and active fragments and variants thereof, DNA and RNA sequences encoding class I-type lysyl-tRNA synthetase (and biological equivalents and fragments thereof), and methods for screening for class I-type lysyl-tRNA synthetase inhibitors for medical and veterinary use. It further provides methods for screening for infection of an organism by microorganisms expressing class I-type lysyl-tRNA synthetase.


REFERENCES:
patent: WO 98/58943 (1998-12-01), None
Bult et al. Complete Genome Sequence of the Methanogenic Archaeon,Methanococcus jannaschii. Science (Aug. 1996) 273:1058-1073.*
GenBank Accession No. C64367 (Sep. 13, 1996).*
Soll et al. Context-dependent anticodon recognition by class I lysyl-tRNA synthetases. Proc Natl Acad Sci U S A Dec. 19, 2000; 97(26): 14224-8.*
Agou, F., et al., Biochemistry 1996, 35:15322-15331.
Chen, J., et al., J. Bacteriology 1994, 176:2699-2705.
Ibba, M., et al., Proc. Natl. Acad. Sci. USA 1997, 94:14383-14388.
Ibba, M., et al., TIBS 1997, 22: 39-42.
Ibba, M., et al., Proc. Natl. Acad. Sci. USA 1999, 96:418-423.
Ibba, M., et al., Science 1997, 278:1119-1122.
Schimmel, P., and Soll, D., Proc. Natl. Acad. Sci. USA 1997, 94: 10007-10009.
Tumbula, D., et al., Genetics 1999, 152:1269-1276.

Affiliated with

Also associated with

Rating

Class I-type lysyl-TRNA synthetase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Class I-type lysyl-TRNA synthetase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Class I-type lysyl-TRNA synthetase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2960900