Class I MHC-restricted T-T hybridomas, and a CD8-transfected BW5

Details Class I MHC-restricted T-T hybridomas, and a CD8-transfected BW5 Class I MHC-restricted T-T hybridomas, and a CD8-transfected BW5

1991-12-24

1994-09-20

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 724, 435 29, 435 691, 4351722, C12N 516

Patent

active

053488784

ABSTRACT:
The present invention provides class I MHC-restricted T-T hybridomas, a novel T-T fusion partner therefor and an assay system that allows for the generation and detection of functional, class I MHC restricted T-T hybridomas. The fusion partner comprises a murine BW5147 cell line that has been transfected with a CD8 gene and stably expresses the CD8 gene product. The BW5147 transfectants are used to generate functional, class I MHC restricted T-T hybridomas which are permissive for lymphokine production. Functional class I MHC-restricted T-T hybridomas exhibiting the capability of producing lymphokines are prepared by fusing BW5147 cells expressing CD8 with T lymphocytes activated in a mixed leukocyte reaction (alloreactive T cells) or stimulated with antigen (antigen-stimulated T lymphocytes) and screening the resulting hybrids for the ability to produce lymphokines in response to appropriate antigenic stimulation. Responsiveness of these clones to alloantigen or to antigen-MHC I complexes can be tested by measuring their production of lymphokines, which provides a sensitive and quantitative assay system.

REFERENCES:
Kanagawa, O., et al. (1989) J. Exp. Med. 170: 901-912.
Ratnofsky, S. E., et al. (1987) id,. 166: 1747-57.
Gu, J. J., et al. (1992) Immunogenetics 36: 283-93.
Kanagawa, O., et al. (1990) Intl. Immunol. 2: 957-64.
Weiner, D. B., et al. (1986) Cell. Immunol. 100: 197-209.
Dembic', Z. et al. (1987) Nature 326: 510-11.
Dembic', Z., et al. (1986) Nature 320: 232-38.
Haas, et al. (1985) Eur. J. Immunol. 15: 755-60.
Moore, et al. (1988) Cell 54: 777-85.
Burgert et al, "Reactivity of VB17a+ CD8+ T Cell Hybrids," J. Exp. Med., 170: 1887-1904 (Dec., 1989).
Whitaker et al, "CT Hybridomas: Tumor Cells Capable of Lysing Virally Infected Target Cells," Journal of Immunology, vol. 129, No. 2, (1982).
Kaufman et al, "Cytotoxic T lymphocyte hybridomas that mediate specific tumor-cell lysis in vitro," Proc. Natl. Acad. Sci., U.S.A., vol. 78, No. 4, 2502-2506 (1981).
Endres et al, "An IL-2 Secreting T Cell Hybridoma That Responds To A Self Class I Histocompatibility Antigen In The H-2D Region," Journal of Immunology, vol. 131, No. 4, pp. 1656-1662 (1983).
Gabert et al, "Reconstitution of MHC Class I Specificity by Transfer of the T Cell Receptor and Lyt-2 Genes," Cell, vol. 50, pp. 545-554 (1987).
Carbone et al, "Remethylation At Sites 5' Of The Murine Lyt-2 Gene In Association With Shutdown of Lyt-2 Expression," Journal of Immunology, 141: 1369-1375, (1988).
Hagiwara et al, "The AKR Thymoma BW5147 Is Able To Produce Lymphokines When Stimulated With Calcium Ionophore and Phorbol Ester," Journal of Immunology, vol. 140, No. 5, pp. 1561-1565 (1988).
Mossmann and Coffman, "TH1 and TH2 Cells: Different Patterns of Lymphokine Secretion Lead to Difference Functional Properties," Ann. Rev. Immunol., 7:145-73 (1989).
MacDonald et al, "Clonal Heterogeneity in the Functional Requirement for Lyt-2/3 Molecules on Cytolytic T Lymphocytes (CTL) . . .", Immunological Rev., vol. 68 (1982).
Silva et al, "RatXMourse T-Cell Hybrids with Inducible Specific Cytolytic Activity," Immunological Rev., vol. 76, 105-129 (1983).
Kappler et al, "Antigen-Inducible, H-2-Restricted, Interleuken-2-Producing T Cell Hybridomas," J. Exp. Med., vol. 153, 1198-1214 (1981).
Rock and Benacerraf, "MHC-Restricted T Cell Activation: Analysis With T Cell Hybridomas," Immunological Rev., vol. 76, pp. 29-57 (1983).
Rock and Benacerraf, "Inhibition of Antigen-Specific T Lymphocyte Activation By Structurally Related Ir Gene-Controlled Polymers," J. Exp. Med., vol. 157, pp. 1618-1634 (1983).
Bierer et al, "The Biological Roles of CD2, CD4 and CD8 in T Cell Activation," Ann. Rev. Immunol., 7: 579-599 (1989).
Rock, K., "Functional T-Cell Hybridomas", Hybridoma Technology in BioScience and Medicine, Timothy A. Springer, ed., Plenum Press, pp. 527-544 (1985).

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