Clarifying E. coli fermentation broths with a combination of ani

Details Clarifying E. coli fermentation broths with a combination of ani Clarifying E. coli fermentation broths with a combination of ani

1995-01-18

1996-09-03

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Separation of micro-organism from culture media

435106, 435814, 210705, 210723, 210732, C12N 102, C12P 1304, B03D 300

Patent

active

055523161

ABSTRACT:
A method which uses a combination of polymeric cationic and an anionic flocculants to clarify an aqueous solution containing cells of a microorganism and parts thereof is described. The anionic flocculant is based upon a copolymer of an alpha-beta unsaturated monomer of an anhydride, a carboxylic acid or salt and an alpha-beta Unsaturated sulfonic acid or salt monomer. The polymeric cationic surfactant is preferably a quaternary ammonium or tertiary amine containing polymer produced from an alpha-beta unsaturated amino monomer. The method is particularly useful for clarifying solutions wherein a bacterium is used to produce an expressed material such as a protein, peptide, or amino acid dissolved in the solution which is to be separated from the cells or cell parts. Particularly preferred is a method wherein the anionic polymer and therafter the cationic polymer are mixed sequentially into the fermentation broth whereby a flocculant mass containing the polymers, cells and parts of cells is allowed to separate from the broth.

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