Circularly permuted fluorescent protein indicators

Details Circularly permuted fluorescent protein indicators Circularly permuted fluorescent protein indicators

2006-06-13

2006-06-13

Murphy, Joseph (Department: 1646)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C530S402000, C435S069700, C435S320100, C435S325000

Reexamination Certificate

active

07060793

ABSTRACT:
Polynucleotides encoding fluorescent indicators, which contain a sensor polypeptide inserted within a fluorescent moiety, are provided, as are polypeptides encoded by such polynucleotides. Also provided are circularly permuted fluorescent polypeptides and polynucleotides encoding the circularly permuted fluorescent polypeptides. In addition, methods of using the fluorescent indicators and the circularly permuted fluorescent polypeptides are provided.

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