Circularly permutated, interaction-activated proteins

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S004000, C506S009000, C424S134100

Reexamination Certificate

active

07544477

ABSTRACT:
Interaction-activated circularly permutated proteins are disclosed that depend for their functional reassembly into the parent protein on the interaction of heterologous polypeptides or other molecules which have been genetically or chemically conjugated to the break-point termini of engineered enzymes. In addition, methods are provided for identifying circularly permutated marker proteins that will optimally reassemble into a functional parent protein, and which are dependent on the association of heterologous interactor domains. The invention is exemplified by circular permutations of a Class A β-lactamase (TEM-1 ofE. coli). Circularly permutated marker proteins that comprise molecular interaction-dependent enzymes particularly find use in (1) cell-based sensors for activation or inhibition of metabolic or signal transduction pathways for high-efficiency, (2) high-throughput screening for agonists/antagonists of the target pathway and in high-throughput mapping of pair-wise protein-protein interactions within and between the proteomes of cells, tissues, and pathogenic organisms, and in (3) cell-based screens for high-throughput selection of inhibitors of any protein-protein interaction.

REFERENCES:
patent: 6270964 (2001-08-01), Michnick et al.
patent: 6294330 (2001-09-01), Michnick et al.
patent: WO 95 29195 (1995-11-01), None
patent: WO 96 30540 (1996-10-01), None
patent: WO 98 34120 (1998-08-01), None
patent: WO 98 44350 (1998-10-01), None
patent: WO 99 28746 (1999-06-01), None
patent: WO 00 07038 (2000-02-01), None
Pieper et al (Biochemistry, 1997, 36, 8767-8774).
Remy et al (Proc. Natl. Acad. Sci. USA, vol. 96, pp. 5394-5399, May 1999).
PDB entry for beta lactamase downloaded Jul. 17, 2007—This citation replaces the citation for item U on the 892 dated Jul. 17, 2007.
Ostermeier, et al, A combinatorial approach to hybrid enzymes independent of DNA homology, Nature Biotechnology, vol. 17, No. 12, pp. 1205-1209, Dec. 1999.
Ostermeier et al., Combinatorial protein engineering by incremental truncation, Proc. Natl. Acad. Sci., vol. 96, pp. 3562-3567, Mar. 1999.
Blackwood and Eisenman,Science(1991) 251:1211.
Defeo-Jones et al.,Nature(1991) 352:251.
Fields and Song,Nature(1989) 340:245.
Chien et al.,Proc. Natl. Acad. Sci. (1991) 88:9578.
Zervos et al.,Cell(1993) 72:223.
Vojtek et al.,Cell(1993) 74:205.
Luban et al.,Cell(1993) 73:1067.
Bartel et al.,Nature Genetics(1996) 2:72.
Fromont-Racine et al.,Nature Genetics(1997) 3:277.
Xu et al.,Proc. Natl. Acad. Sci(1997) 94:12473.
Bartel et al.,Biotechniques(1993) 14:920.
Krebber et al.,J. Mol. Biol. (1997) 268:607.
Cubitt et al.,Trends Biochem(1995) 20:448.
Rossi et al.,Proc. Natl. Acad. Sci(1997) 94:8405.
Pelletier et al.,Proc. Natl. Acad. Sci. (1998) 95:12141.
Karimova et al.,Proc. Natl. Acad. Sci. (1998) 95:5752.
Pieper et al.Biochemistry, (1997) 36: 8767-8774.
U.S. Appl. No. 09/526,106, filed Mar. 15, 2000, Balint, B.F., et al.
Pelletier, et al, Protein Engineering. (1997), 10:89.
Johnson, et al, Proc Natl Acad Sci. (1994) 91:10340-10344.
Voet, D. and Voet, J.G.,Biochemistry, Second Edition, New York: John Wiley and Sons, 1995, pp. 123-128 (Section 6-3A) and p. 230, column 2, first paragraph.
Wehrman, T., et al., “Protein-protein interactions monitored in mammalian cells via complementation of b-lactamase enzyme fragments,” PNAS, Mar. 19, 2002, 99(6):3469-3474.
Lu, Zhijian et al.; “Expression of Thioredoxin Random Peptide Libraries on theEscherichia coliCell Surface as Functional Fusions to Flagellin: A System Designed for Exploring Protein-Protein Interactions”; 1995,Biotechnology, vol. 13, pp. 366-372.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Circularly permutated, interaction-activated proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Circularly permutated, interaction-activated proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Circularly permutated, interaction-activated proteins will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4148951

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.