Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2001-01-16
2009-06-09
Low, Christopher S F (Department: 1639)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S004000, C506S009000, C424S134100
Reexamination Certificate
active
07544477
ABSTRACT:
Interaction-activated circularly permutated proteins are disclosed that depend for their functional reassembly into the parent protein on the interaction of heterologous polypeptides or other molecules which have been genetically or chemically conjugated to the break-point termini of engineered enzymes. In addition, methods are provided for identifying circularly permutated marker proteins that will optimally reassemble into a functional parent protein, and which are dependent on the association of heterologous interactor domains. The invention is exemplified by circular permutations of a Class A β-lactamase (TEM-1 ofE. coli). Circularly permutated marker proteins that comprise molecular interaction-dependent enzymes particularly find use in (1) cell-based sensors for activation or inhibition of metabolic or signal transduction pathways for high-efficiency, (2) high-throughput screening for agonists/antagonists of the target pathway and in high-throughput mapping of pair-wise protein-protein interactions within and between the proteomes of cells, tissues, and pathogenic organisms, and in (3) cell-based screens for high-throughput selection of inhibitors of any protein-protein interaction.
REFERENCES:
patent: 6270964 (2001-08-01), Michnick et al.
patent: 6294330 (2001-09-01), Michnick et al.
patent: WO 95 29195 (1995-11-01), None
patent: WO 96 30540 (1996-10-01), None
patent: WO 98 34120 (1998-08-01), None
patent: WO 98 44350 (1998-10-01), None
patent: WO 99 28746 (1999-06-01), None
patent: WO 00 07038 (2000-02-01), None
Pieper et al (Biochemistry, 1997, 36, 8767-8774).
Remy et al (Proc. Natl. Acad. Sci. USA, vol. 96, pp. 5394-5399, May 1999).
PDB entry for beta lactamase downloaded Jul. 17, 2007—This citation replaces the citation for item U on the 892 dated Jul. 17, 2007.
Ostermeier, et al, A combinatorial approach to hybrid enzymes independent of DNA homology, Nature Biotechnology, vol. 17, No. 12, pp. 1205-1209, Dec. 1999.
Ostermeier et al., Combinatorial protein engineering by incremental truncation, Proc. Natl. Acad. Sci., vol. 96, pp. 3562-3567, Mar. 1999.
Blackwood and Eisenman,Science(1991) 251:1211.
Defeo-Jones et al.,Nature(1991) 352:251.
Fields and Song,Nature(1989) 340:245.
Chien et al.,Proc. Natl. Acad. Sci. (1991) 88:9578.
Zervos et al.,Cell(1993) 72:223.
Vojtek et al.,Cell(1993) 74:205.
Luban et al.,Cell(1993) 73:1067.
Bartel et al.,Nature Genetics(1996) 2:72.
Fromont-Racine et al.,Nature Genetics(1997) 3:277.
Xu et al.,Proc. Natl. Acad. Sci(1997) 94:12473.
Bartel et al.,Biotechniques(1993) 14:920.
Krebber et al.,J. Mol. Biol. (1997) 268:607.
Cubitt et al.,Trends Biochem(1995) 20:448.
Rossi et al.,Proc. Natl. Acad. Sci(1997) 94:8405.
Pelletier et al.,Proc. Natl. Acad. Sci. (1998) 95:12141.
Karimova et al.,Proc. Natl. Acad. Sci. (1998) 95:5752.
Pieper et al.Biochemistry, (1997) 36: 8767-8774.
U.S. Appl. No. 09/526,106, filed Mar. 15, 2000, Balint, B.F., et al.
Pelletier, et al, Protein Engineering. (1997), 10:89.
Johnson, et al, Proc Natl Acad Sci. (1994) 91:10340-10344.
Voet, D. and Voet, J.G.,Biochemistry, Second Edition, New York: John Wiley and Sons, 1995, pp. 123-128 (Section 6-3A) and p. 230, column 2, first paragraph.
Wehrman, T., et al., “Protein-protein interactions monitored in mammalian cells via complementation of b-lactamase enzyme fragments,” PNAS, Mar. 19, 2002, 99(6):3469-3474.
Lu, Zhijian et al.; “Expression of Thioredoxin Random Peptide Libraries on theEscherichia coliCell Surface as Functional Fusions to Flagellin: A System Designed for Exploring Protein-Protein Interactions”; 1995,Biotechnology, vol. 13, pp. 366-372.
Balint Robert F.
Her Jeng-Horng
Gross Christopher M
KaloBios, Inc.
Low Christopher S F
Townsend and Townsend / and Crew LLP
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