Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2011-03-01
2011-03-01
Ketter, James S (Department: 1636)
Chemistry: molecular biology and microbiology
Vector, per se
C435S069100, C435S252330, C435S235100
Reexamination Certificate
active
07897380
ABSTRACT:
Circular nucleic acid vectors that provide for persistently high levels of protein expression are provided. The circular vectors of the subject invention are characterized by being devoid of expression-silencing bacterial sequences, where in many embodiments the subject vectors include a unidirectional site-specific recombination product hybrid sequence in addition to an expression cassette. Also provided are methods of using the subject vectors for introduction of a nucleic acid, e.g., an expression cassette, into a target cell, as well as preparations for use in practicing such methods. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications. Also provided is a highly efficient and readily scalable method for producing the vectors employed in the subject methods, as well as reagents and kits/systems for practicing the same.
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Chen Zhi-Ying
Kay Mark A.
Alcamo Elizabeth A.
Bozicevic Field & Francis LLP
Field Bret E.
Ketter James S
The Board of Trustees of the Leland Stanford Junior University
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