Circular extinction contrast imaging microscope

Details Circular extinction contrast imaging microscope Circular extinction contrast imaging microscope

2007-11-06

2007-11-06

Amari, Alessandro (Department: 2872)

Optical: systems and elements

Compound lens system

Microscope

C359S386000, C356S365000

Reexamination Certificate

active

10968834

ABSTRACT:
Systems and methods for producing circular extinction (CE) contrast images of anisotropic samples. Microscope systems for determining circular extinction (CE), the differential transmission of left and right circularly polarized light resulting from circular dichroism (CD) of an anisotropic sample, include mechanically driven optical components and an image detector such as a monochromatic CCD camera to detect light intensities. In one aspect, optical components include a tunable filter, a rotatable linear polarizer and a variable retarder. The tunable filter is adjustable to provide light at a specific desired wavelength. The linear polarizer is adjustable to provide linearly polarized light with a specific wave vector, and the variable retarder is adjustable to produce near perfect circular polarized light at every selected wavelength. For example, in one aspect, the variable retarder includes a linear birefringent plate tiltable around one of its eigenmodes perpendicular to the wave vector of polarized light. The plate may be controllably tilted so that it functions as a perfect λ/4 plate at each wavelength.

REFERENCES:
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patent: 6924893 (2005-08-01), Oldenbourg et al.
Reiko Kuroda, “A solid-state dedicated circular dichroism spectrophotometer; Development and application”, Review of Scientific Instruments, 2001, vol. 72, No. 10, pp. 3802-3810.
Colin Nuckolls et al., “Circular Dichorism and UV-Visible Absorption Spectra of the Langmulr-Blodgett Films of an Aggregating Helicene”, J. Am. Chem. Soc., 1998, vol. 120, pp. 8656-8660.

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