Circular DNA molecule having a conditional origin of...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S252300, C435S252330

Reexamination Certificate

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10268948

ABSTRACT:
A circular DNA molecule, useful for gene therapy, comprising at least one nucleic acid sequence of interest, characterised in that the region allowing the replication thereof has an origin of replication with a functionality in a host cell that requires the presence of at least one specific protein foreign to said host cell. A method for preparing same, cells incorporating said DNA molecules and uses thereof in gene therapy are also described.

REFERENCES:
patent: 4190495 (1980-02-01), Curtiss, III
patent: 4654307 (1987-03-01), Morgan
patent: 4761367 (1988-08-01), Edgell et al.
patent: 5198343 (1993-03-01), DeGryse
patent: 5434065 (1995-07-01), Mahan et al.
patent: 5510099 (1996-04-01), Short et al.
patent: 5656481 (1997-08-01), Baetge et al.
patent: 5693622 (1997-12-01), Wolff et al.
patent: 5700657 (1997-12-01), Beaudry et al.
patent: 5714323 (1998-02-01), Ohshima et al.
patent: 5773246 (1998-06-01), Keene et al.
patent: 5851808 (1998-12-01), Elledge et al.
patent: 5859208 (1999-01-01), Fiddes et al.
patent: 5874259 (1999-02-01), Szybalski
patent: 5955056 (1999-09-01), Short et al.
patent: 5985644 (1999-11-01), Roseman et al.
patent: 6254874 (2001-07-01), Mekalanos et al.
patent: 6573100 (2003-06-01), Seeber et al.
patent: WO95/30762 (1995-11-01), None
patent: WO96/01899 (1996-01-01), None
Panicker et al., J. Bacteriol. 162 (2) 584-590, 1985.
W. French Anderson, Human gene therapy, NATURE, vol. 392, pp. 25-28.
Dalyot et al., Efficient Transfer Of The Complete Human Beta-Globin Gene Into Human And Mouse Hemopoeitic Cells Via SV40 Pseudovirions, Gene Transfer & Gene Therapy 47-56 (1989).
Filutowicz et al., J. Cell Sci. Suppl. 7, 15-31, 1987.
Flensburg et al., J. Mol. Biol. 195:439-445, 1987.
Gluzman, SV40-Transformed Simian Cells Support the Replication of Early SV40 Mutants, Cell, 23:175-182 (1981).
Herrero et al., Transposon Vectors Containing Non-Antibiotic Resistance Selection Markers for Cloning and Stable Chromosomal Insertion of Foreign Genes in Gram-Negative Bacteria, Journal of Bacteriology, 172(11):6557-6567 (1990).
Inuzuka et al., An initiator protein for plasmid R6K DNA replication Mutations affecting the copy-number control, FEBS Letters 228(1):7-11 (1988).
Larsen et al., Low-copy-number plasmid-cloning vectors amplifiable by derepression of an inserted foreign promoter, Gene 28:45-54 (1984).
Metcalf et al., Use of the rep technique for allele replacement to construct newEscherichia colihosts for maintenance of R6Ky origin plasmids at different copy numbers, Gene 138:1-7 (1994).
Posfai et al., NAR 22(12):2392-2398, 1994.
Rusconi et al., A novel expression assay to study transcriptional activators, Gene 89:211-221 (1990).
June Rothman Scott, “Regulation of Plasmid Replication,”Microbiological Reviews, Mar. 1984, vol. 48, No. 1, p. 1-23.
Summers et al., Multimerization of High Copy Number Plasmids Causes Instability: ColE1 Encodes a Determinant Essential for Plasmid Monomerization and Stability, Cell 36:1097-1103 (1984).
Vasavada et al., A contingent replication assay for the detection of protein-protein interactions in animal cells, Proc. Natl. Acad. Sci., USA 88:10686-10690 (1991).
Winnacker, E.L.,From Genes to Clones, pp. 132-138, 1987.
Koons et al., “Characterization of pPvu1, the autonomous plasmid from Proteus vulgaris that carries the genes of the Pvu11 restriction-modiciation system,” Gene, 157:73-79, 1995.
Baum et al., “Novel Cloning Vectors forBacillus thuringiensis,” Applied and Environmental Microbiology, 56:3420, 1990.
Penfold et al., “An improved suicide vector for construction of chromosomal insertion mutations in bacteria,” Gene, 18:145-146, 1992.
Bergemann et al., “Excision of specific DNA-sequences from integrated retroviral vectors via site-specific recombination,” Nucleic Acids Research, 23(21):4451-4456, 1995.
Gage et al., “A Cell-Free Recombination System for Site-Specific Integration of Multigenic Shuttle Plasmids into the Herpes Simplex Virus Type 1 Genome,” Journal of Virology, 66(9):5509-5515, 1992.
Hanzlik et al., “A small plasmid for recombination-based screening,” Gene, 122:171-174, 1992.
Metzger et al., “Conditional site-specific recombination in mammalian cells using a ligand-dependent chimeric Cre recombinase,” Proc. Natl. Acad. Sci. USA, 92:6991-6995, 1995.
Stewart et al., “Plasmids for recombination-based screening,” Gene, 106:97-101, 1991.
Kelley et al., “Conformational Changes Induced by Integration Host Factor at Origin γ of R6K and Copy Number Control,” The Journal of Biological Chemistry, 266(24):15924-15937, 1991.

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