Chymopapain allergen and method

Drug – bio-affecting and body treating compositions – In vivo diagnosis or in vivo testing – Magnetic imaging agent

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424 88, 424 94, 435184, 435212, 435219, A61K 3935, A61K 4900, C12N 999, C12N 950

Patent

active

044990650

ABSTRACT:
A chymopapain derivative having the enzymatic activity reduced by at least 95% while retaining the original allergenic activity of at least 90% is suitable for skin testing to detect allergic hypersensitivity to chymopapain and for treating patients exhibiting allergic hypersensitivity to chymopapain. The enzymatic activity can be blocked by reacting thiol groups of the enzyme with and iodoacetic acid, bromoacetic acid, or a salt, ester or amide derivative thereof, under conditions which block the undesirable enzymatic activity while retaining the desired allergenic activity.

REFERENCES:
patent: 4374926 (1983-02-01), Stern
patent: 4439423 (1984-03-01), Smith
White et al., Principles of Biochemistry, Fourth Edition, 1968, p. 239.
Ebata et al., Biochemical and Biophysical Research Communications, vol. 9, No. 2, pp. 173-178 (1962).
Ebata et al., Biochim. Biophys. Acta, vol. 118, pp. 201-203 (1966).

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