Chromosome characterization using single fluorescent dye

Details Chromosome characterization using single fluorescent dye Chromosome characterization using single fluorescent dye

1994-03-25

1995-05-23

Redding, David A.

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-o

436172, 436800, 435 6, 209577, 209578, 2504612, 935 19, 935 77, G01N 2164

Patent

active

054181690

ABSTRACT:
Chromosomes are characterized by fluorescent emissions from a single fluorescent dye that is excited over two different wavelengths. A mixture containing chromosomes is stained with a single dye selected from the group consisting of TOTO and YOYO and the stained chromosomes are placed in a flow cytometer. The fluorescent dye is excited sequentially by a first light having a wavelength in the ultraviolet range to excite the TOTO or YOYO to fluoresce at a first intensity and by a second light having a wavelength effective to excite the TOTO or YOYO dye to fluoresce at a second intensity. Specific chromosomes may be identified and sorted by intensity relationships between the first and second fluorescence emissions.

REFERENCES:
Hirons, G. T., and Crissman, H. A. Evaluation of two new fluorochromes, TOTO and YOYO, for DNA content analysis in cells and chromosomes by flow cytometry. 16 Congress of the International Society for Analytical Cytology. 21-26 Mar. 1993. Colorado Springs, Colo. 1993.
Hays S. Rye et al., "Fluorometric Assay Using Dimeric Dyes For Double-and Single-Stranded DNA and RNA With Picogram Sensitivity," Analytical Biochemistry 208, pp. 144-150 (1993).
J. W. Gray et al., "High-Speed Chromosome Sorting," Science, 23:323 (1987).

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