Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1993-12-21
1995-11-28
Fleisher, Mindy B.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 914, C12N 1564, C12N 1566
Patent
active
054707277
ABSTRACT:
The present invention provides compositions and methods for inserting a copy of a heterologous gene into the chromosome of a host cell such as E. coli through the use of a chromosomal transfer DNA, a circular, non-self-replicating DNA carrying a site-specific recombination site such as lambda att P. The host cell chromosome contains a second site-specific recombination site. When the chromosomal transfer DNA is introduced into the host cell, expression of an integration enzyme such as integrase causes the integration of the chromosomal transfer DNA into the host cell chromosome at the second recombination site.
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Mascarenhas Desmond
Olson Pamela S.
Carter Philip W.
Celtrix Pharmaceuticals Inc.
Fleisher Mindy B.
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