Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1991-07-12
1993-08-24
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 792, 435 25, 435188, 436815, 436904, C12Q 128, C12N 996
Patent
active
052388174
ABSTRACT:
A substrate for detecting peroxidase activity in a peroxidase-based assay which includes a substantially aqueous solution of at least a 1-naphthol derivative and tetramethylbenzidine is described.
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patent: 4849342 (1989-07-01), Ben-Michael
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Conyers et al, Anal. Biochem., vol. 192, pp. 207-211 (1991).
Bos et al, J. of Immunoassay, vol. 2, Nos. 3 and 4, pp. 187-204, 1981.
Brand et al., BioTechniques, 8(1): 58-60 (1990), describes a comparison of the performance and stability of two precipitating substrate systems for immunoblotting, namely, TMB and DAB.
Kobayashi and Tashima, "Visualization of Antigen on Nitrocellulose Membrane by the Oxidative Coupling Reaction of N,N'-Dimethyl-p-phenylenediamine and 4-chloro-1-naphthol," Analytical Biochemistry 183, 9-12 (1989).
Kirkegaard & Perry Laboratories, Inc. 1993 Catalog, "Substrates for Use with Peroxidase-Labeled Antibodies," pp. 47-52.
De Jong et al., Histochemical Journal 17:1119-1130 (1985) describes various visualization methods for peroxidase and alkaline phosphatase activity with respect to sensitivity, stability of chromogen solutions and final precipitates, background staining, and localization properties.
Nachlas et al., J. Histochem. Cytochem., 6: 445-456 (1958), describes 4-amino-1-N,N-dimethylnaphthylamine useful as a reagent for the Nadi reaction which can provide a histochemical method for either cyctochrome oxidase (G-Nadi) or peroxidase (M-Nadi).
Kirkegaard & Perry Labs, Product Catalog, "Substrates for Use with Peroxidase-labeled Antibodies", pp. 29-31 (1990), describes immunoblotting with tetramethylbenzidine (TMB).
Kirkegaard & Perry Labs, Product Catalog, pp. 5-6 (Summer 1990) provides information concerning peroxidase substrates for immunoassays on membranes.
McKimm-Breschkin, J. Immunological Methods 135: 277-280 (1990), describes the use of TMB for solid phase immunoassays.
Modern Photographic Processing, vol. 2, pp. 453-553 (1979), describes the nature of color development and use of color developing agents such as p-phenyleneddiamines.
Bobrow Mark N.
Litt Gerald J.
E. I. Du Pont de Nemours and Company
Mohamed Abdel A.
Wityshyn Michael G.
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