Chromogenic substrate to peroxidase enzymes

Details Chromogenic substrate to peroxidase enzymes Chromogenic substrate to peroxidase enzymes

1992-08-03

1993-06-01

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

436800, C12Q 128

Patent

active

052158900

ABSTRACT:
A novel chromogenic substrate to peroxidase enzymes is provided which is comprised of a mixture of an adduct formed from a hydrozone and a dienophile, and an aromatic nucleophile. The mixture undergos an oxidative coupling in the presence of peroxidase enzymes and peroxides forming a purple indamine dye. The mixture is also stable and unaffected by oxygen of the air or by hydrogen peroxide.

REFERENCES:
Akiba et al. Chem. Abst. vol. 78 (1973) p. 111,192u.
George et al. Chem. Abstr. vol. 71 (1969) p. 22,111e.
Dunn-Chem. Abst. vol. 102 (1985) p. 6364t.

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