Chromogenic substrate to peroxidase enzymes

Details Chromogenic substrate to peroxidase enzymes Chromogenic substrate to peroxidase enzymes

1988-02-16

1991-08-27

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 12, 435 14, 435192, 436904, 534649, 534753, 534770, 534784, 534789, 534795, C12Q 128

Patent

active

050432699

ABSTRACT:
A novel chromogenic substrate to peroxidase enzymes is provided which is comprised of a mixture of a colorless mixture of two compounds, such as, 3-methyl-2-benzothiazolinone hydrazone and 2-hydroxy-2,4,6-cycloheptatrienone, which undergo an oxidative coupling in the presence of peroxidase and hydrogen peroxide forming a purple indamine dye. Under certain circumstances the dye will precipitate out from the buffered solution and thus provide a permanent record of the determination. The mixture is also stable and unaffected by oxygen of the air or by hydrogen peroxide.

REFERENCES:
Geoghegan, W., "Adaptation of the Ngo-Lenhoff Peroxidase Assay for Solid Phase ELISA"; J. of Immunol. Methods, 60 (1983), 61-68.
Ngo, T., "A Sensitive and Versatile Chromogenic Assay for Peroxidase . . . ", Anal. Biochem., 105 (1980), 389-397.
Kahn, V., "Tropolone as a Substrate for Horseradish Peroxidase", Phytochemistry, v. 24, No. 5 (1985), 909-913.
Schmid, P., "Differential Substrate Specificity of Isoperoxidases . . . ", Angew. Botanik, 62 (1988), 161-168.

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