Chromogenic substrate to peroxidase enzymes

Details Chromogenic substrate to peroxidase enzymes Chromogenic substrate to peroxidase enzymes

1993-05-28

1995-07-11

Fan, Jane T.

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

548161, C07D41712

Patent

active

054322855

ABSTRACT:
A novel chromogenic substrate to peroxidase enzymes is provided which is comprised of a mixture of an adduct formed from a hydrozone and a dienophile, and an aromatic nucleophile. The mixture undergos an oxidative coupling in the presence of peroxidase enzymes and peroxides forming a purple indamine dye. The mixture is also stable and unaffected by oxygen of the air or by hydrogen peroxide.

REFERENCES:
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CA 84(9):59288n Akiba et al. 1975.
CA 84(9):59287m Akiba et al. 1975.
CA 83(21):177580d Akiba et al. 1975.
Akiba, et al. Bull. Chem. Soc. Jpn. 48, 1975, pp. 3270-3273, p. 3262.
Akiba et al. Chem. Lett, 1975 347-350.
Akiba et al. CA 78:111192u, 1973.
Elslager et al. J. Med. Chem. 7, 1964 pp. 493, 496.

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