Chromogenic substrate for determination of amylase activity

Details Chromogenic substrate for determination of amylase activity Chromogenic substrate for determination of amylase activity

1976-04-14

1977-05-24

Tanenholtz, Alvin E.

Textiles: fiber preparation

195101, 1951035S, G01N 3300, G01N 3114

Patent

active

040253929

ABSTRACT:
An improved chromogenic substrate for .alpha.-amylase assays is synthesized by reacting amylose with Cibachron Blue F3GA, sodium sulfate and trisodium phosphate and incubating the resultant dyed amylose substrate with a buffer containing calcium chloride. The new substrate is more sensitive than commercially available chromogenic substrates in determining the .alpha.-amylase activity of the enzymes of sweet potato, tobacco, and human saliva.

REFERENCES:
patent: 3679661 (1972-07-01), Babson
Ewen, "Synthesis of Cibachron Blue F3GA-Amylose with Increased Sensitivity for Determination of Amylase Activity" Clinica Chemica Acta., 47, (1973) pp. 233-248.
Klein et al., Clinical Chem. vol. 16, No. 1, (1970) pp. 32-38.
Lang, "Biochemists' Handbook", D. Van Nostrand Co., Inc., Princeton, N.J. 1961 p. 232.

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