Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1987-11-09
1989-04-25
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 28, 435188, 435810, 436 66, G01N 3353, G01N 3372, C12Q 128, C12N 996
Patent
active
048247847
ABSTRACT:
A chromogenic solution for enzyme immunoassay. A stabilizing agent is added to chromogenic solutions containing chromogen of the hydrogen donor type. The stabilizing agent is an alkylhydroxylamine, preferably N,N-diethylhydroxylamine. The N-N diethylhydroxylamine retards degradation of the solution of the chromogen in solvent and is particularly effective in retarding discoloration of a chromogen-solvent solution placed in storage over an extended period of time. The alkylhydroxylamine preferably in the form of N,N-diethylhydroxylamine has the additional property that it does not adversely affect the activity of enzymes employed in enzyme immunoassays. It also does not interfere with binding specificity or reactivity of antibodies.
REFERENCES:
patent: 3791932 (1974-02-01), Schuurs et al.
patent: 3888629 (1975-06-01), Bagshawe
patent: 4067690 (1978-01-01), Cuisia et al.
patent: 4157280 (1979-06-01), Halbert et al.
patent: 4169012 (1979-09-01), Dawson et al.
patent: 4248965 (1981-02-01), Mochida et al.
patent: 4256833 (1981-03-01), Ali et al.
patent: 4273868 (1981-06-01), Walter
patent: 4287300 (1981-09-01), Gibbons et al.
patent: 4299916 (1981-11-01), Litman et al.
patent: 4340395 (1982-07-01), Magers et al.
patent: 4361648 (1982-11-01), Shuenn-tzong
patent: 4366241 (1982-12-01), Tom et al.
patent: 4385114 (1983-05-01), Guthlein et al.
patent: 4386224 (1983-05-01), Deetman
patent: 4391904 (1983-07-01), Litman et al.
patent: 4407943 (1983-10-01), Cole et al.
patent: 4444879 (1984-04-01), Foster et al.
patent: 4446232 (1984-05-01), Liotta
patent: 4448882 (1984-05-01), Brodbeck et al.
patent: 4458014 (1984-07-01), Ebersole
patent: 4486530 (1984-12-01), David et al.
patent: 4503143 (1985-03-01), Gerber et al.
patent: 4525452 (1985-06-01), Jones et al.
patent: 4540659 (1985-09-01), Litman et al.
patent: 4615972 (1986-10-01), Gallacher
patent: 4632901 (1986-12-01), Valkirs et al.
patent: 4727019 (1988-02-01), Valkirs et al.
P. K. Nakane et al., "Peroxidase-Labeled Antibody: A New Method of Conjugation", J. of Histochemistry and Cytochemistry, vol. 22, pp. 1084-1091 (1974).
P. Nakane, "Preparation and Standardization of Enzyme-Labeled on Conjugates", Immunoassays in the Clinical Laboratory, Alan R. Liss, Inc., pp. 81-87 (1979).
E. Engvall, "Immunochemical Techniques", Methods in Enzymology, Part A, H. V. Vunakis et al., eds. Academic Press, pp. 430-432 (1980).
M. Uotila et al., "Two-Site Sandwich Enzyme Immunoassay with Monoclonal Antibodies To Human Alpha Fetoprotein", J. Immunological Methods, 42, pp. 11-15 (1981).
E. S. Bos et al., "3,3',5,5'-Tetramethylbenzidine as an Ames Test Negative Chromogen for Horse-Radish Peroxidase In Enzyme-Immunoassay", J. of Immunoassay, 2(3&4), pp. 187-204 (1981).
S. Fujita, "Reducing Agents for Quinanes. N,N-Diethylhydroxylamine and Others", Yuki Gosei Kogaku Kyokaishai, 37, pp. 960-966 (1979).
Hoffer Florina B.
Hygeia Sciences, Incorporated
Josephs Barry D.
Kepplinger Esther M.
Kirby, Jr. John P.
LandOfFree
Chromogenic solution for immunoassay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Chromogenic solution for immunoassay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chromogenic solution for immunoassay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1195569