Chromogenic reactive for determining the iron content and the ir

Details Chromogenic reactive for determining the iron content and the ir Chromogenic reactive for determining the iron content and the ir

1986-12-29

1989-03-07

Richman, Barry S.

Chemistry: analytical and immunological testing

Metal or metal containing

Present in biological fluids

436 84, 436175, 436910, G01N 3320, G01N 3349

Patent

active

048106569

ABSTRACT:
The present invention relates to a chromogenic reagent for determining the iron content and the iron-binding capacity of serum, comprising chromazurol B, a buffer for keeping the pH at 4.5-5, a surface-active substance in a concentration of at least 500 mg/l, a salt suitable for imparting an ionic strength expressed in terms of NaCl concentration of at least 100 g/l and an aminoacid suitable for selectively binding the Cu.sup.2+ ions, in a concentration expressed in terms of glycine concentration ranging between 1 and 40 g/l.

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patent: 4407962 (1983-10-01), Tabacco et al.
patent: 4567150 (1986-01-01), Tabacco et al.
patent: 4588695 (1986-05-01), Takano et al.
Tabacco et al., "Clin. Chim. Acta", 114; pp. 287-290, 1981.
Stephens et al., "Amer. Chem. Soc., Book and J. Div.", vol. 46, No. 6, pp. 692-696, May 1974.

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