Chromogenic enzyme substrates

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Having -c- – wherein x is chalcogen – bonded directly to...

Reexamination Certificate

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Details

C548S452000, C548S465000, C514S412000, C514S415000

Reexamination Certificate

active

07323488

ABSTRACT:
This invention provides novel chromogenic enzyme substrates which are indoxyl β-D-ribofuranosides. A process for their production is provided. Methods for detecting β-D-ribofuranosidase activity are given. The advantages of these novel compounds includes: detecting β-D-ribofuranosidase activity with high sensitivity and low substrate concentrations and use with other enzyme indicators in situations where a plurality of enzyme activities is to be visualized simultaneously e.g. for identifying bacteria growth on solid growth media. The synthesis and use of 5-bromo-4-chloro-3-indolyl-β-D-ribofuranoside is exemplified.

REFERENCES:
patent: 5364767 (1994-11-01), Flowers et al.
patent: 6350588 (2002-02-01), Roth et al.
patent: 2 770 538 (1997-11-01), None
patent: WO 97/31008 (1997-08-01), None
Berlin et al (1996): STN International HCAPLUS database, Columbus (OH), accession No. 1996: 734811.
1. Masao Shiozaki , “Synthesis of 4′,8-dihydroxyisoflavon-7-yl D-arabinofuranoside” Exploratory Chemistry Research Laboratories pp. 1477-1742 dated 1999.
2. Berlin et al. XP-002271582 In Situ Color Detection of L-Arabinofuranosidase, a “No-Background” Reporter Gene, with 5-Bromo-3-indolyl-a -L-arabinofuranoside pp. 171-174 (dated 1996).
3. XP-002214084 (abstract only).
4. XP-002214085 (abstract only).

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