Chromogenic compounds, and use thereof as enzyme substrates

Details Chromogenic compounds, and use thereof as enzyme substrates Chromogenic compounds, and use thereof as enzyme substrates

2007-05-29

2007-05-29

Weber, Jon (Department: 1657)

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

C435S024000

Reexamination Certificate

active

10541490

ABSTRACT:
The present invention relates to novel chromogenic substrates which are used to detect aminopeptidase activity in microorganisms or to determine whether at least one bacterium belongs to the Gram-positive group or to the Gram-negative group according to the color thereof. The invention also relates to culture media containing such substrates, to the use of the substrates or media for the detection of aminopeptidase activities and/or the differentiation of Gram-positive bacteria from Gram-negative bacteria and to methods of use.The aforementioned novel substrates have the formula below:in which:R1is nothing or an alkyl, allyl or aryl group,R2consists of at least one amino acid, preferably alanine,R3, R4, R5and R6consist, independently of one another, of H— or —O-alkyl, preferably —O—CH3,R7consists of H, O—CH3, alkyl or halogen,R8consists of H or Cl, andn is an integer corresponding to 0 or 1.The invention is particularly suitable for use in the field of diagnostics.

REFERENCES:
patent: 4457866 (1984-07-01), Karges et al.
patent: 0 270 946 (1988-06-01), None
patent: 0 656 421 (1995-06-01), None
patent: 2 659 982 (1991-09-01), None
patent: WO 98/04735 (1998-02-01), None
patent: WO 99/09207 (1999-02-01), None
patent: WO 99/38995 (1999-08-01), None
patent: WO 01/09372 (2001-02-01), None
S. Rapposch et al.; “Influence of Fluorescence of Bacteria Stained with Acridine Orange on the Enumeration of Microorganisms in Raw Milk”;J. Dairy Sci.; vol. 83, No. 12; 2000; pp. 2753-5758.
A. Giorgio et al.; “Detection of Microorganisms in Clinical Specimens Using Slides Prestained with Acridine Orange (AOS)”;Microbiologica; vol. 12, No. 1; 1989; pp. 97-100.
Nadine Costes et al.; “Synthesis and Cytotoxic and Antitumor Activity of Benzo[b ]pyrano[3,2- i h l ]acridin-7-one Analogues of Acronycine”;J. Med. Chem.; vol. 43, No. 12; 2000; pp. 2395-2402.
O. Okwumabua et al.; “Evaluation of chemiluminescent DNA probe assay for the rapid confirmation ofListeria monocytogenes”; Res. Microbiol.; vol. 143, No. 2; 1992; pp. 183-189.
Thomas Schelhorn et al.; “Review, Reinvestigation of the Binding of Proflavine to DNA. Is Intercalation the Dominant Binding Effect?”;Cellular and Molecular Biology; vol. 38, No. 4; 1992; pp. 345-365.
Adrien Albert;The Acridines: Their Preparation, Physical, Chemical, and Biological Properties and Uses; 1966; second edition; pp. 29-55.
A. Campbell et al.; “5-Alkylacridines. Part I. Synthesis of 5-Methylacridine and Certain Substituted Analogues”;J. Chem. Soc.; 1958; pp. 1145-1149.

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