Liquid purification or separation – With means to add treating material – Chromatography
Patent
1990-05-02
1992-08-04
Therkorn, Ernest G.
Liquid purification or separation
With means to add treating material
Chromatography
2105021, 210635, 210656, 502404, 502439, 536 183, 536120, B01D 1508
Patent
active
051356503
ABSTRACT:
Highly compressible chromatographic stationary phase particles such as agarose beads are made rigid to a degree suitable for use in HPLC, and nonporous to proteins by one of two procedures. The first involves shrinking the beads with the use of an organic solvent in which the agarose bead will neither dissolve nor swell to collapse the porosity, followed by crosslinking the bead surfaces inside the collapsed pores to fix the pores in their collapsed state. The second involves filling the pores (without shrinkage of the beads) with a polymerizable substance which grafts to the pore surface, and performing the graft polymerization. The invention also extends to rigid beads, which are rendered deformable to a limited degree by coating the surface with a polymer. Finally, porous rigid beads are rendered nonporous by polymerizing a polymerizable material inside the pores in the same manner as the porous compressible beads.
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Hjerten Stellan
Liao Jia-li
Bio-Rad Laboratories, Inc.
Therkorn Ernest G.
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