Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Patent
1997-08-13
1999-12-21
Till, Terrence R.
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
204602, 204601, G01N 2726
Patent
active
060044438
ABSTRACT:
A novel free-fluid electrophoresis system applicable to both "analytical" and "preparative" separations combines the advantages of electrophoresis with those of automated liquid chromatography. The system attains plug flow of sample molecules within a separation column that has a sufficiently small diameter to avoid uncorrected convective dispersions and a sufficiently large diameter to ensure adequate preparative throughput. Sample molecules are introduced by liquid chromatographic-type injection mechanisms and moved to the separation column by means of a highly accurate, low-pressure pumping system. A novel pressure detector monitors pressure across the column during the electrophoretic separation. When the pressure across the column is zero, electroosmotic flow within the column exactly balances pump-induced flow so that there is no lateral dispersion of the separating sample molecules (i.e., plug flow is achieved). The pressure detector is monitored by a computer that controls either the pump and/or the electrophoretic power supply to maintain balance between the pressure-driven flow and the electroosmotically driven flow; alternatively carefully controlled departures from the balance can be maintained to increase throughput. The separated sample molecules can be optically detected within the separation column. When the separated sample molecules leave the separation column, they can be collected using fraction collection equipment similar to that used in liquid chromatography. The use of programmed nonuniform voltage and temperature fields allow for practically unlimited scale-up potential.
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Rhodes Percy H.
Snyder Robert S.
Noguerola Alex
Till Terrence R.
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