Chromatography columns with continuous beds formed in situ from

Liquid purification or separation – With means to add treating material – Chromatography

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2105021, 210635, 210656, B01D 1508

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059354291

ABSTRACT:
A chromatography column with a continuous solid bed spanning the cross section of the column, the bed containing channels large enough for hydrodynamic flow, is prepared by polymerizing a mixture of monomers and ammonium sulfate in an aqueous solution. The monomers include a monofunctional monomer such as a vinyl, allyl, acrylic, or methacrylic compound, and a polyfunctional monomer (i.e., a crosslinker), the total monomer concentration being in the range of 10% to 20% by weight, the mole ratio of crosslinker to total monomer being in the range of 0.3 to 0.4, and the ammonium sulfate having a concentration in the range of 0.4 M to 0.8 M. Functional groups to impart specialized separation capabilities, notably anion and cation exchange, can be included in the monomer mixture.

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