Chromatography

Details Chromatography Chromatography

1979-04-17

1981-03-24

Cintins, Ivars C.

Liquid purification or separation

Processes

Chromatography

210502, 2101982, 55 67, 252316, 435288, 435182, 23230B, 424 15, B01D 1508

Patent

active

042578841

ABSTRACT:
Semipermeable microcapsules are employed as a medium to chromatographically separate mixtures of solutes. The microcapsules comprise membranes having a selected upper limit of permeability and contain a filler material capable of maintaining the membranes in a distended position. In use, the capsules are packed in a column and equilibrated with the solvent of the mixture to be separated. Components in the mixture are separated when passed through the column on the basis of their molecular diameter. The material is well suited for resolving components of a mixture of solutes in the 100-5000 molecular weight range.
In another embodiment, a specific binding substance such as an antibody is confined within capsule membranes permeable to the antibody's complementary antigen to produce a material useful in affinity chromatography.

REFERENCES:
patent: 3471261 (1969-10-01), Patterson
patent: 3859228 (1975-01-01), Morishita et al.
patent: 3947352 (1976-03-01), Cuatrecasas et al.
patent: 3985840 (1975-10-01), Hofacker
patent: 4143201 (1979-03-01), Miyashiro et al.
"The Microencapsulation of Hemoglobin Solutions: Techniques of Production and Characterization," R. D. Moss, PHD Thesis, May 1975.

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