Chromatographically purifying proteolytic procoagulant enzyme fr

Details Chromatographically purifying proteolytic procoagulant enzyme fr Chromatographically purifying proteolytic procoagulant enzyme fr

1982-06-23

1984-07-24

Marantz, Sidney

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

210656, 435212, 435217, 435219, 435226, 435815, 436543, 436547, 436813, C12Q 138, C12Q 156

Patent

active

044618336

ABSTRACT:
A chromatographic procedure for the purification of a proteolytic procoagulant enzyme from extracts of human and animal tumors. The extracts are sequentially contacted with a first benzamide affinity chromatographic resin, an agarose filtration gel, a second benzamide affinity chromatographic resin and a phenyl-sepharose hydrophobic chromatographic resin. The resulting enzyme is capable of producing anti-procoagulant antibody which, which used in an immunoassay, is diagnostic for malignancy.

REFERENCES:
patent: 3515641 (1970-06-01), Whitaker
patent: 4252902 (1981-02-01), Fujii
patent: 4264738 (1981-04-01), Stepanov
patent: 4374926 (1983-02-01), Stern
patent: 4393140 (1983-07-01), Schutt

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