Chromatographic supports having an immobilized flocculating agen

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic

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210656, 210661, 210723, 210807, 422 59, 436524, 436526, 436531, 436536, 436538, 436539, 436541, 436500, 436824, 530412, 530413, 530418, G01N 33544, G01N 33546, G01N 33551, G01N 33553

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active

053025326

ABSTRACT:
Disclosed is a primary chromatographic support containing an immobilized flocculating agent for use as a separation media for a secondary support consisting of microparticles used in affinity separation and heterogeneous immunoassays. In a specific embodiment, a flocculating agent such as polyethyleneimine is immobilized on a chromatographic resin packed in a column. The column so formed is then used to trap microparticles having an affinity ligand or binder for the ligand affixed thereon. Such microparticles are used as a solid support for the affinity reaction involved in a variety of immunoassay formats.

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