Chromatographic supports having an immobilized flocculating agen

Details Chromatographic supports having an immobilized flocculating agen Chromatographic supports having an immobilized flocculating agen

1989-04-14

1994-04-12

Saunders, David

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is organic

210656, 210661, 210723, 210807, 422 59, 436524, 436526, 436531, 436536, 436538, 436539, 436541, 436500, 436824, 530412, 530413, 530418, G01N 33544, G01N 33546, G01N 33551, G01N 33553

Patent

active

053025326

ABSTRACT:
Disclosed is a primary chromatographic support containing an immobilized flocculating agent for use as a separation media for a secondary support consisting of microparticles used in affinity separation and heterogeneous immunoassays. In a specific embodiment, a flocculating agent such as polyethyleneimine is immobilized on a chromatographic resin packed in a column. The column so formed is then used to trap microparticles having an affinity ligand or binder for the ligand affixed thereon. Such microparticles are used as a solid support for the affinity reaction involved in a variety of immunoassay formats.

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