Chromatographic process for the copurification of chondroitinase

Details Chromatographic process for the copurification of chondroitinase Chromatographic process for the copurification of chondroitinase

1994-04-22

1996-06-11

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

435814, 435815, 435873, 530415, 530416, C12N 988, C07K 118, C07K 122

Patent

active

055255000

ABSTRACT:
A chromatographic process for the copurification of chondroitinase proteins useful in ocular surgery for non-surgical disruption of chondroitin sulfate, the molecule which mediates the attachment between the retina and vitreous body in the human eye. The process involves the use of ion exchange resins in conjunction with an affinity elution with chondroitin sulfate to afford copurification of the proteins.

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patent: 5292509 (1994-03-01), Hageman
Yamagata et al. (1968) J. Biol. Chem., 243(7), 1523-1535.
Pharmacia (1974) Affinity Chromatography, Principles and Methods, Pharmacia Fine Chemicals, Uppsala, Sweden, pp. 56-57.
Kitamikado et al. (1975) Appl. Microbiol., 29(3), 414-421.
Sato et al, (1986) Agric. Biol. Chem., 50(4), 1057-1059.
Sato et al, (1994) Appl. Microbiol. Biotechnol., 41(1), 39-46.

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