Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Insulin; related peptides
Patent
1995-04-28
1997-04-15
Lukton, David
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
Insulin; related peptides
530303, 530304, 530412, 514 3, A61K 3828
Patent
active
056210736
ABSTRACT:
A process is described for obtaining insulin, which is virtually free from proteases and/or insulin acetylated at position A9, by chromatography in aqueous, buffered solvents which contain water-miscible organic solvents, on lipophilically modified silica gel, wherein the dissolving buffer contains acetone or acetonitrile.
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patent: 5245008 (1993-09-01), Dickhardt et al.
European Search Report, EP 92 12 1255, May 24, 1994.
B.S. Welinder et al., "Separation, Isolation and Characterization of the Four Monoiodinated Insulin Tracers Using Reversed-Phase High-Performance Liquid Chromatography," Journal of Chromatography, 265 (1983) pp. 301-309.
E.P. Kroeff et al., "Production Scale Purification of Biosynthetic Human Insulin by Reversed-Phase High-Performance Liquid Chromatography," Journal of Chromatography, 461 (1989) pp. 45-61.
W.S. Hancock et al., "Biochemical Applications of Preparative Liquid Chromatography," in Preparative Liquid Chromatography, ed. by B.A. Bidlingmeyer, Journal of Chromatography LIbrary, vol. 38, pp. 203-233.
Jean River et al., Journal of Chromatography, vol. 268: 112-119 (1983).
"Reverse-phase high-performance liquid chromatography of insulin Resolution and recovery in relation to column geometry and buffer components", Benny S. Welinder et al., Journal of Chromatography, 361:357-367 (1986).
Dickhardt Rainer
Gr afe Claudia
Unger Bernhard
Hoechst Aktiengesellschaft
Lukton David
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