Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1981-01-12
1982-08-31
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435180, 435201, 435205, 435234, 435815, C12N 1112, C12N 926, C12N 992
Patent
active
043473229
ABSTRACT:
Enzyme purification is carried out by contacting an impure liquid enzyme preparation containing enzyme and soluble impurities with an ion exchange material in a column to adsorb both the enzyme and impurities by the ion exchange material, adding an additional amount of the impure liquid enzyme preparation whereby the soluble impurities therein are preferentially adsorbed by the ion exchange material and the adsorbed enzyme is displaced from the ion exchange material to produce a purified liquid enzyme preparation containing higher enzyme activity than before purification. The purified enzyme is more highly adsorbed by ion exchange material when immobilizing the enzyme.
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"Encyclopedia of Chemical Technology", Kirk-Othmer, Second Edition, vol. 5, Chromatography, pp. 413-429, 442-449, John Wiley & Sons, New York 1964.
Johnson Richard A.
Lloyd Norman E.
Conzett Robert A.
Kornutik Richard
Nabisco Brands Inc.
Naff David M.
Wyzan Henry S.
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