Chromatographic method with micellar on-column enrichment

Liquid purification or separation – Processes – Chromatography

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210635, 2101982, 436 89, 436161, 436178, B01D 1508

Patent

active

051356570

ABSTRACT:
On-column enrichment is provided for a sample having both polar and nonpolar analytes by mixing the sample with surfactant in a polar solvent. The surfactant causes the nonpolar analytes to be incorporated in micelles that dissolve in the polar solvent. The concentration of surfactant is maintained at a level sufficient to maintain the micelles as the mixture is introduced onto the head of a chromatographic column. Once sample introduction is completed, the mixture is diluted so that the surfactant concentration drops below that required to maintain the micelles, which thus break up. The analytes are then separated using reverse-phase gradient elution.

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