Chromatographic method and means

Details Chromatographic method and means Chromatographic method and means

1984-08-31

1985-10-01

Adee, John

Liquid purification or separation

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Material

210635, 2101982, 210656, 436161, 502402, 502403, B01D 1508

Patent

active

045444854

ABSTRACT:
A novel packing material is provided for liquid chromatographic columns, having a maximum pore size around 80 angstroms, a hydrophobic coating on the inner surfaces adapted to absorb hydrophobic analytes, and a hydrophilic coating on the outer surfaces adapted to reject proteins in the analyte sample. A novel method is provided for making the packing material, wherein the packing base is coated, inside and out, with a hydrophilic substance, a hydrophobic substance is bonded to the hydrophilic substance, and the hydrophobic substance is selectively removed from the external surfaces by treatment with an enzyme of a size too large to penetrate into the pores.

REFERENCES:
patent: 3598245 (1971-08-01), Determann et al.
patent: 3917527 (1975-11-01), Shaltiel
patent: 4061591 (1977-12-01), Oliver
patent: 4298500 (1981-11-01), Abbott
patent: 4340483 (1982-07-01), Lukas et al.
patent: 4376047 (1983-03-01), Pohl

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