Liquid purification or separation – With means to add treating material – Chromatography
Patent
1986-03-17
1988-04-26
Therkorn, Ernest G.
Liquid purification or separation
With means to add treating material
Chromatography
210290, 210635, 210656, 435219, 435803, 435815, 436501, 530396, 530413, 530417, B01D 1508
Patent
active
047403063
ABSTRACT:
A chromatographic column and process for isolating or purifying steroid hormone or cell membrane receptors using the column are provided in which the column contains at least three resin layers between the inlet and the outlet ends of the column, the layer closest the inlet being a strong cationic exchange resin, the middle layer being a matrix containing a triazine dye that will bind proteases and proteins with dinucleotide fold conformations, and the layer closest the outlet being a weak anionic exchange resin, the ratio of the volumes of the resin layers being 1:2:1.
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Kirsch Ted M.
Litwack Gerald
Temple University - of the Commonwealth System of Higher Educati
Therkorn Ernest G.
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